Literature DB >> 6374895

Adenovirus E1a gene product expressed at high levels in Escherichia coli is functional.

B Ferguson, N Jones, J Richter, M Rosenberg.   

Abstract

The human type C adenovirus E1a 13S messenger RNA encodes a gene product, that positively regulates the transcription of viral genes and certain cellular genes and is involved in the transformation of primary mammalian cells. The E1a gene product was expressed at high levels in Escherichia coli. In a Xenopus oocyte microinjection assay, the purified Escherichia coli-produced protein activated the E1a-responsive adenovirus E3 promoter and functioned as efficiently as the E1a gene itself.

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Year:  1984        PMID： 6374895     DOI： 10.1126/science.6374895

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  51 in total

1.  Biological function of the retinoblastoma protein requires distinct domains for hyperphosphorylation and transcription factor binding.

Authors:  Y Qian; C Luckey; L Horton; M Esser; D J Templeton
Journal:  Mol Cell Biol       Date:  1992-12       Impact factor: 4.272

2.  Nonfunctional mutants of the retinoblastoma protein are characterized by defects in phosphorylation, viral oncoprotein association, and nuclear tethering.

Authors:  D J Templeton; S H Park; L Lanier; R A Weinberg
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205

3.  E1A gene products stimulate in vitro transcription from the adenovirus early region 4 promoter by enhancing a stable preinitiation complex.

Authors:  T Imai; H Kawaguchi; T Wada; H Handa
Journal:  Virus Genes       Date:  1991-01       Impact factor: 2.332

Review 4.  Expression and interactions of human adenovirus oncoproteins.

Authors:  P A Boulanger; G E Blair
Journal:  Biochem J       Date:  1991-04-15       Impact factor: 3.857

5.  Activation in vitro of RNA polymerase II and III directed transcription by baculovirus produced E1A protein.

Authors:  G Patel; N C Jones
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

6.  Fusion of adenovirus E1A to the glucocorticoid receptor by high-resolution deletion cloning creates a hormonally inducible viral transactivator.

Authors:  D M Becker; S M Hollenberg; R P Ricciardi
Journal:  Mol Cell Biol       Date:  1989-09       Impact factor: 4.272

7.  Phosphorylation in vitro of Escherichia coli-produced 235R and 266R tumor antigens encoded by human adenovirus type 12 early transformation region 1A.

Authors:  L A Lucher; P M Loewenstein; M Green
Journal:  J Virol       Date:  1985-10       Impact factor: 5.103

8.  Synthesis in Escherichia coli of human adenovirus type 12 transforming proteins encoded by early region 1A 13S mRNA and 12S mRNA.

Authors:  D Kimelman; L A Lucher; K H Brackmann; J S Symington; M Ptashne; M Green
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

9.  Pentapeptide nuclear localization signal in adenovirus E1a.

Authors:  R H Lyons; B Q Ferguson; M Rosenberg
Journal:  Mol Cell Biol       Date:  1987-07       Impact factor: 4.272

10.  Studies of the expression of the Wiskott-Aldrich syndrome protein.

Authors:  D M Stewart; S Treiber-Held; C C Kurman; F Facchetti; L D Notarangelo; D L Nelson
Journal:  J Clin Invest       Date:  1996-06-01       Impact factor: 14.808
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