Immunocytochemical localizations of cytosolic and mitochondrial glutamic oxaloacetic transaminase isozymes in rat brain.

The localizations of cytosolic (s-) and mitochondrial (m-) glutamic oxaloacetic transaminase (GOT) were examined by immunocytochemical methods using specific antibodies. Staining of s-GOT-like immunoreactivity was seen in periglomerular cells of the olfactory bulb, and basket, stellate cells of the cerebellum, and second layer cells of the neocortex. On the other hand, m-GOT-like immunoreactivity was found in mitral cells and glomerular regions of the olfactory bulb and deep Golgi cells of the cerebellum. These different distributions of s- and m-GOT isozymes suggest that these isozymes are available as markers of glutamergic or aspartergic neurons.