Literature DB >> 6370095

[Assay of rabbit anti-thermolabile enterotoxin antibodies from Escherichia coli by an immunoenzyme technic].

Y Germani, B Dassy, G Le Gonidec, J L Guesdon.   

Abstract

The titration of rabbit anti-Escherichia coli heat-labile enterotoxin antibodies by an immunosorbent assay (ELISA) generally uses a GM1 coating step to lend specificity to the ELISA reaction at a time when the method is tempered by a lack of purified E. coli toxin. We have developed an adsorption method of the toxin to the polystyrene in order to simplify the technique. Three different immunosorbent preparations were tested to determine which of them yielded the most sensitive results.

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Year:  1984        PMID: 6370095

Source DB:  PubMed          Journal:  Ann Immunol (Paris)        ISSN: 0300-4910


  2 in total

1.  Comparison of two GM1-erythrocyte assays to detect heat-labile Escherichia coli enterotoxin in stool specimens.

Authors:  Y Germani; J L Guesdon; L Phalente; E Begaud; J P Moreau
Journal:  J Clin Microbiol       Date:  1988-05       Impact factor: 5.948

2.  GM1 erythroimmunoassay for detection and titration of Escherichia coli heat-labile enterotoxin.

Authors:  Y Germani; E Bégaud; J L Guesdon; J P Moreau
Journal:  J Clin Microbiol       Date:  1986-11       Impact factor: 5.948

  2 in total

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