Development and characterization of a monoclonal antibody (Aby 6C6) that distinguishes medullary from cortical thymocytes.

A hybridoma (6C6) producing antibodies with specificities to canine thymocyte surface antigens was produced using standard fusion techniques. The Aby 6C6 has affinity for medullary thymocytes, bone marrow cells, T- and B-peripheral lymphocytes, and monocytes. The Aby 6C6 appears to have 2 heavy chains with different molecular weights, an isoelectric focusing pattern consistent with monoclonality, and is an immunoglobulin G2b antibody with a kappa light chain.