Development and characterization of a hybridoma-derived antibody (Aby 1A1) with specificity to canine thymocytes and peripheral T lymphocytes.

A hybridoma (1A1)-producing antibody with reactivity to canine T-lymphocyte surface antigens was produced, using standard fusion techniques. Antibody secreted from this hybridoma was tested for specific anti-canine lymphocyte activity, using an enzyme-linked immunosorbent assay, an indirect-fluorescent antibody assay, and cytotoxicity assay. Antibody 1A1 reacted with canine T lymphocytes, but not with B lymphocytes, and with brain tissue and connective tissue. The antibody was also characterized. Its heavy chain had a molecular weight (mol wt) of 80,000 and its light chain was 28,000 mol wt. The Aby 1A1 is a monoclonal immunoglobulin M with a kappa light chain, and it binds poorly to Staphylococcus A.