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Literature DB >> 6368570

Identification of coated vesicles in Saccharomyces cerevisiae.

Abstract

Clathrin-coated vesicles were found in yeast, Saccharomyces cerevisiae, and enriched from spheroplasts by a rapid procedure utilizing gel filtration on Sephacryl S-1000. The coated vesicles (62-nm diam) were visualized by negative stain electron microscopy and clathrin triskelions were observed by rotary shadowing. The contour length of a triskelion leg was 490 nm. Coated vesicle fractions contain a prominent band with molecular weight of approximately 185,000 when analyzed by SDS PAGE. The presence of coated vesicles in yeast cells suggests that this organism will be useful for studying the function of clathrin-coated vesicles.

Entities: Chemical Species

Mesh：

Substances：
Clathrin

Year: 1984 PMID： 6368570 PMCID： PMC2113006 DOI： 10.1083/jcb.98.1.341

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

18 in total

1. Coated vesicles from pig brain: purification and biochemical characterization.

Authors: B M Pearse
Journal: J Mol Biol Date: 1975-09-05 Impact factor: 5.469

2. Clathrin: a unique protein associated with intracellular transfer of membrane by coated vesicles.

Authors: B M Pearse
Journal: Proc Natl Acad Sci U S A Date: 1976-04 Impact factor: 11.205

3. Morphological evidence for de novo formation of plasma membrane from coated vesicles in exponentially growing cultured plant cells.

Authors: W W Franke; W Herth
Journal: Exp Cell Res Date: 1974-12 Impact factor: 3.905

4. Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli.

Authors: R C Valentine; B M Shapiro; E R Stadtman
Journal: Biochemistry Date: 1968-06 Impact factor: 3.162

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

6. Quantification of Coomassie Blue stained proteins in polyacrylamide gels based on analyses of eluted dye.

Authors: C Fenner; R R Traut; D T Mason; J Wikman-Coffelt
Journal: Anal Biochem Date: 1975-02 Impact factor: 3.365

7. Protoplasmic organization of hyphal tips among fungi: vesicles and Spitzenkörper.

Authors: S N Grove; C E Bracker
Journal: J Bacteriol Date: 1970-11 Impact factor: 3.490

8. Involvement of vesicle coat material in casein secretion and surface regeneration.

Authors: W W Franke; M R Lüder; J Kartenbeck; H Zerban; T W Keenan
Journal: J Cell Biol Date: 1976-04 Impact factor: 10.539

9. Functions of coated vesicles during protein absorption in the rat vas deferens.

Authors: D S Friend; M G Farquhar
Journal: J Cell Biol Date: 1967-11 Impact factor: 10.539

10. Lysosomes and GERL in normal and chromatolytic neurons of the rat ganglion nodosum.

Authors: E Holtzman; A B Novikoff; H Villaverde
Journal: J Cell Biol Date: 1967-05 Impact factor: 10.539

27 in total

1. Identification of a putative yeast homolog of the mammalian beta chains of the clathrin-associated protein complexes.

Authors: T Kirchhausen
Journal: Mol Cell Biol Date: 1990-11 Impact factor: 4.272

2. Dissociation and reassembly of soybean clathrin.

Authors: R E Wiedenhoeft; G W Schmidt; B A Palevitz
Journal: Plant Physiol Date: 1988-02 Impact factor: 8.340

3. Receptor-Mediated Endocytosis in Plants is Energetically Possible.

Authors: M J Saxton; R W Breidenbach
Journal: Plant Physiol Date: 1988-04 Impact factor: 8.340

4. Viability of clathrin heavy-chain-deficient Saccharomyces cerevisiae is compromised by mutations at numerous loci: implications for the suppression hypothesis.

Authors: A L Munn; L Silveira; M Elgort; G S Payne
Journal: Mol Cell Biol Date: 1991-08 Impact factor: 4.272

Review 5. Genetic analysis of clathrin function in yeast.

Authors: G S Payne
Journal: J Membr Biol Date: 1990-06 Impact factor: 1.843

6. Ultracytochemical localization of X-prolyl-dipeptidyl (amino)peptidase in microglobules and endoplasmic membranes accumulated in pep4-3 mutant of Saccharomyces cerevisiae.

Authors: J Vorísek
Journal: Histochemistry Date: 1986

10. Suppressors of clathrin deficiency: overexpression of ubiquitin rescues lethal strains of clathrin-deficient Saccharomyces cerevisiae.

Authors: K K Nelson; S K Lemmon
Journal: Mol Cell Biol Date: 1993-01 Impact factor: 4.272