Literature DB >> 6367744

Cloning a cDNA for the lysosomal alpha-glucosidase.

A Konings, P Hupkes, R Versteeg, G Grosveld, A Reuser, H Galjaard.   

Abstract

Messenger RNA was isolated from monkey testes and size-fractionated on sucrose gradients. In vitro translation of these mRNA fractions resulted in nascent, labeled alpha-glucosidase that could be precipitated with anti human alpha-glucosidase antiserum. A cDNA library was constructed from the most enriched fraction. The library was screened with cDNA made from mRNA obtained from immunoselected polysomes. Five cross-hybridizing clones were isolated and identified by their selection of alpha-glucosidase mRNA, as shown by hybrid released translation and further by their ability to hybridize with DNA from human chromosome 17, on which the gene coding for acid alpha-glucosidase is located.

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Year:  1984        PMID： 6367744     DOI： 10.1016/0006-291x(84)91645-0

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  8 in total

Review 1.  Human biochemical genetics of enzyme proteins in the new age of molecular genetics.

Authors:  D M Swallow; D A Hopkinson
Journal:  J Inherit Metab Dis       Date:  1986       Impact factor: 4.982

2.  Molecular cloning of a cDNA for human alpha-L-fucosidase.

Authors:  H Fukushima; J R de Wet; J S O'Brien
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

Review 3.  Trafficking of lysosomal enzymes in normal and disease states.

Authors:  S Kornfeld
Journal:  J Clin Invest       Date:  1986-01       Impact factor: 14.808

4.  Regional mapping of the human gene for lysosomal alpha-glucosidase by in situ hybridization.

Authors:  D J Halley; A Konings; P Hupkes; H Galjaard
Journal:  Hum Genet       Date:  1984       Impact factor: 4.132

5.  Isolation of a cDNA for human acid alpha-glucosidase and detection of genetic heterogeneity for mRNA in three alpha-glucosidase-deficient patients.

Authors:  F Martiniuk; M Mehler; A Pellicer; S Tzall; G La Badie; C Hobart; A Ellenbogen; R Hirschhorn
Journal:  Proc Natl Acad Sci U S A       Date:  1986-12       Impact factor: 11.205

6.  Subcellular distribution of acid alpha-glucosidase in fibroblasts and of antigenically cross-reactive material in Pompe's disease fibroblasts.

Authors:  T Iwamasa; K Nashiro; T Ohshita; I Matsuda
Journal:  Histochem J       Date:  1986 Nov-Dec

7.  The chromosomal localization of human beta-galactosidase revisited: a locus for beta-galactosidase on human chromosome 3 and for its protective protein on human chromosome 22.

Authors:  H J Sips; H A de Wit-Verbeek; J de Wit; A Westerveld; H Galjaard
Journal:  Hum Genet       Date:  1985       Impact factor: 4.132

8.  Primary structure and processing of lysosomal alpha-glucosidase; homology with the intestinal sucrase-isomaltase complex.

Authors:  L H Hoefsloot; M Hoogeveen-Westerveld; M A Kroos; J van Beeumen; A J Reuser; B A Oostra
Journal:  EMBO J       Date:  1988-06       Impact factor: 11.598
  8 in total

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