| Literature DB >> 6366059 |
R M Fauve, H Jusforgues, B Hevin.
Abstract
Granulomas were induced by injecting polyacrylamide beads into subcutaneous pouches created by divulsion of the dorsal skin of mice. More than 10(7) phagocytic cells (60% macrophages, 40% polymorphonuclear cells) could be recovered from this granuloma. The separation of the phagocytic cells can be achieved either following sedimentation in Percoll or following the incubation of cells on plastic petri dishes. Phagocytosis of zymosan was observed in macrophages maintained in vitro for 1 month in Eagle's serum-free medium.Entities:
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Year: 1983 PMID： 6366059 DOI： 10.1016/0022-1759(83)90442-8
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303