| Literature DB >> 6365591 |
V M Blinov, V V Gutorov, N G Holodilov, A A Iljichev, V A Karginov, N N Mikrjukov, V A Mordvinov, I V Nikonov, N A Petrov, I H Urmanov.
Abstract
The initiation sites of the Galleria mellonella L. nuclear polyhedrosis virus (G.m. NPV) DNA replication were revealed. For this purpose SCLd 135 cells permitting the G.m. NPV productive reproduction were transformed by the recombinant plasmids containing the viral genome individual fragments in pRSF 2124 and pBR 322 vectors. It was revealed that 2 of the 32 recombinant plasmids can autonomously replicate in the eucaryotic cells. According to the Maxam-Gilbert method the DNA G.m. NPV fragment (1300 bp) primary structure of pHBR plasmid was determined. The structure analysis revealed the typical regulator signals as in the replicons. The possible regulation mechanism of the DNA G.m. NPV synthesis initiation was supposed.Entities:
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Year: 1984 PMID： 6365591 DOI： 10.1016/0014-5793(84)80137-4
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124