Literature DB >> 6364970

Improved method for detection of glycosidases in bacterial colonies.

N F Paoni, R L Arroyo.   

Abstract

An assay has been developed to detect bacterial glycosidases in colonies grown on the surface of agar plates. Advantages of this technique over previously described methods include elimination of the need for replica plating, better visualization of chromagenic reaction products, and a simple permeabilization step to enable better penetration by chromagenic substrates.

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Year:  1984        PMID： 6364970      PMCID： PMC239639          DOI： 10.1128/aem.47.1.208-209.1984

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  1 in total

1.  Direct immunoassay for detecting Escherichia coli colonies that contain polypeptides encoded by cloned DNA segments.

Authors:  D J Kemp; A F Cowman
Journal:  Proc Natl Acad Sci U S A       Date:  1981-07       Impact factor: 11.205
  1 in total
  3 in total

1.  Analysis of the critical sites for protein thermostabilization by proline substitution in oligo-1,6-glucosidase from Bacillus coagulans ATCC 7050 and the evolutionary consideration of proline residues.

Authors:  K Watanabe; K Kitamura; Y Suzuki
Journal:  Appl Environ Microbiol       Date:  1996-06       Impact factor: 4.792

2.  Cloning and expression in Escherichia coli of an extremely thermostable oligo-1,6-glucosidase gene from Bacillus thermoglucosidasius.

Authors:  K Watanabe; H Iha; A Ohashi; Y Suzuki
Journal:  J Bacteriol       Date:  1989-02       Impact factor: 3.490

3.  Cloning and expression of a thermostable exo-alpha-1,4-glucosidase gene from Bacillus stearothermophilus ATCC12016 in Escherichia coli.

Authors:  Y Takii; K Daimon; Y Suzuki
Journal:  Appl Microbiol Biotechnol       Date:  1992-11       Impact factor: 4.813
  3 in total

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