Literature DB >> 6364140

Nuclease protection analysis of ribonucleoprotein complexes: use of the cytotoxic ribonuclease alpha-sarcin to determine the binding sites for Escherichia coli ribosomal proteins L5, L18, and L25 on 5S rRNA.

P W Huber, I G Wool.   

Abstract

A rapid and convenient method has been devised to determine the binding sites for proteins on RNA. The procedure is an adaptation of one used to map DNA-protein complexes by protection against nuclease digestion. The method uses the cytotoxic ribonuclease alpha-sarcin, which hydrolyzes purines in both single- and double-stranded regions of RNA. It has been authenticated by confirming the binding sites for the Escherichia coli ribosomal proteins L18 and L25 on 5S rRNA and its value has been established by identifying the attachment site for protein L5. The procedure should be useful for the analysis of other ribonucleoprotein complexes.

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Year:  1984        PMID： 6364140      PMCID： PMC344668          DOI： 10.1073/pnas.81.2.322

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  30 in total

1.  Affinity binding of Escherichia coli ribosomal proteins to immobilized RNA.

Authors:  H R Burrell; J Horowitz
Journal:  FEBS Lett       Date:  1975-01-01       Impact factor: 4.124

2.  Effects of some proteins that inactivate the eukaryotic ribosome.

Authors:  C Fernandez-Puentes; D Vazquez
Journal:  FEBS Lett       Date:  1977       Impact factor: 4.124

3.  Specific cleavage of ribosomal RNA caused by alpha sarcin.

Authors:  D G Schindler; J E Davies
Journal:  Nucleic Acids Res       Date:  1977-04       Impact factor: 16.971

4.  Isolation and characterization of 5S RNA-protein complexes from Bacillus stearothermophilus and Escherichia coli ribosomes.

Authors:  J R Horne; V A Erdmann
Journal:  Mol Gen Genet       Date:  1972

5.  The sequence of steps in the attachment of 5-S RNA to cores of Escherichia coli ribosomes.

Authors:  R S Yu; H G Wittmann
Journal:  Biochim Biophys Acta       Date:  1973-10-26

6.  Identification of the nucleotide sequences involved in the interaction between Escherichia coli 5 RNA and specific 50 S subunit proteins.

Authors:  P N Gray; G Bellemare; R Monier; R A Garrett; G Stöffler
Journal:  J Mol Biol       Date:  1973-06-15       Impact factor: 5.469

7.  5S RNA secondary structure.

Authors:  G E Fox; C R Woese
Journal:  Nature       Date:  1975-08-07       Impact factor: 49.962

8.  The mode of action of alpha sarcin and a novel assay of the puromycin reaction.

Authors:  A N Hobden; E Cundliffe
Journal:  Biochem J       Date:  1978-01-15       Impact factor: 3.857

9.  Mapping adenines, guanines, and pyrimidines in RNA.

Authors:  H Donis-Keller; A M Maxam; W Gilbert
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

10.  ALPHA SARCIN, A NEW ANTITUMOR AGENT. II. FERMENTATION AND ANTITUMOR SPECTRUM.

Authors:  J C JENNINGS; B H OLSON; V ROGA; A J JUNEK; D M SCHUURMANS
Journal:  Appl Microbiol       Date:  1965-05
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  23 in total

1.  Structure of Escherichia coli ribosomal protein L25 complexed with a 5S rRNA fragment at 1.8-A resolution.

Authors:  M Lu; T A Steitz
Journal:  Proc Natl Acad Sci U S A       Date:  2000-02-29       Impact factor: 11.205

2.  Two crystal forms of helix II of Xenopus laevis 5S rRNA with a cytosine bulge.

Authors:  Y Xiong; M Sundaralingam
Journal:  RNA       Date:  2000-09       Impact factor: 4.942

3.  Ribosomal protein L5 has a highly twisted concave surface and flexible arms responsible for rRNA binding.

Authors:  T Nakashima; M Yao; S Kawamura; K Iwasaki; M Kimura; I Tanaka
Journal:  RNA       Date:  2001-05       Impact factor: 4.942

4.  Isolation and nucleotide sequence of the Aspergillus restrictus gene coding for the ribonucleolytic toxin restrictocin and its expression in Aspergillus nidulans: the leader sequence protects producing strains from suicide.

Authors:  B Lamy; J Davies
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

5.  Two distinct structural elements of 5S rRNA are needed for its import into human mitochondria.

Authors:  Alexandre Smirnov; Ivan Tarassov; Anne-Marie Mager-Heckel; Michel Letzelter; Robert P Martin; Igor A Krasheninnikov; Nina Entelis
Journal:  RNA       Date:  2008-02-26       Impact factor: 4.942

6.  Two zinc finger proteins from Xenopus laevis bind the same region of 5S RNA but with different nuclease protection patterns.

Authors:  M S Sands; D F Bogenhagen
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

7.  Stem-loop IV of 5S rRNA lies close to the peptidyltransferase center.

Authors:  O Dontsova; V Tishkov; S Dokudovskaya; A Bogdanov; T Döring; J Rinke-Appel; S Thamm; B Greuer; R Brimacombe
Journal:  Proc Natl Acad Sci U S A       Date:  1994-05-10       Impact factor: 11.205

8.  Structure of 5S rRNA within the Escherichia coli ribosome: iodine-induced cleavage patterns of phosphorothioate derivatives.

Authors:  O V Shpanchenko; O A Dontsova; A A Bogdanov; K H Nierhaus
Journal:  RNA       Date:  1998-09       Impact factor: 4.942

9.  A specific 31-nucleotide domain of U1 RNA directly interacts with the 70K small nuclear ribonucleoprotein component.

Authors:  C C Query; R C Bentley; J D Keene
Journal:  Mol Cell Biol       Date:  1989-11       Impact factor: 4.272

10.  The NMR structure of Escherichia coli ribosomal protein L25 shows homology to general stress proteins and glutaminyl-tRNA synthetases.

Authors:  M Stoldt; J Wöhnert; M Görlach; L R Brown
Journal:  EMBO J       Date:  1998-11-02       Impact factor: 11.598
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