Literature DB >> 6362894

Stabilization of the maturation promoting factor (MPF) from Xenopus laevis oocytes. Protection against calcium ions.

J Hermann, R Bellé, J Tso, R Ozon.   

Abstract

Maturation promoting factor (MPF) activity was recovered from progesterone-matured Xenopus oocytes cytosol, fractionated by polyethylene glycol-20,000 or ethanolic precipitation. An improved stabilization of the biological activity was obtained by adding adenosine 5'-O-(3-thiotriphosphate) (ATP-gamma-S) (50 microM) to the preparation buffers. Neither Ca2+ ions nor calmodulin inhibit the partially purified MPF.

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Year:  1983        PMID： 6362894     DOI： 10.1016/0045-6039(83)90106-9

Source DB:  PubMed          Journal:  Cell Differ        ISSN： 0045-6039


  5 in total

1.  Metabolic cooperation following fusion of starfish ootid and primary oocyte restores meiotic-phase-promoting activity.

Authors:  P Guerrier; I Neant
Journal:  Proc Natl Acad Sci U S A       Date:  1986-07       Impact factor: 11.205

2.  In vivo effects of microinjected alkaline phosphatase and its low molecular weight substrates on the first meiotic cell division in Xenopus laevis oocytes.

Authors:  J Hermann; O Mulner; R Bellé; J Marot; J Tso; R Ozon
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205

3.  Metaphase protein phosphorylation in Xenopus laevis eggs.

Authors:  M J Lohka; J L Kyes; J L Maller
Journal:  Mol Cell Biol       Date:  1987-02       Impact factor: 4.272

4.  Involvement of protein phosphatases 1 and 2A in the control of M phase-promoting factor activity in starfish.

Authors:  A Picard; J P Capony; D L Brautigan; M Dorée
Journal:  J Cell Biol       Date:  1989-12       Impact factor: 10.539

5.  Okadaic acid mimics a nuclear component required for cyclin B-cdc2 kinase microinjection to drive starfish oocytes into M phase.

Authors:  A Picard; J C Labbé; H Barakat; J C Cavadore; M Dorée
Journal:  J Cell Biol       Date:  1991-10       Impact factor: 10.539
  5 in total

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