Literature DB >> 6362667

Large scale purification and structural properties of yeast aspartyl-tRNA synthetase.

B Lorber, D Kern, A Dietrich, J Gangloff, J P Ebel, R Giegé.   

Abstract

A large scale purification procedure of baker's yeast aspartyl-tRNA synthetase is described which yields more than 200 mg pure protein starting from 30 Kg of wet commercial cells. The synthetase is an alpha 2 dimer of Mr = 125,000 +/- 5,000 which can be crystallized (J. Mol. Biol. 138, 1980, 129-135). The enzyme has an elongated shape with a Stokes radius of 50 A and a frictional ratio of 1.5. The synthetase has a tendency to aggregate but methods are described where this effect is overcome.

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Year:  1983        PMID： 6362667     DOI： 10.1016/0006-291x(83)91569-3

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  13 in total

1.  The peculiar architectural framework of tRNASec is fully recognized by yeast AspRS.

Authors:  J Rudinger-Thirion; R Giegé
Journal:  RNA       Date:  1999-04       Impact factor: 4.942

2.  Efficient mischarging of a viral tRNA-like structure and aminoacylation of a minihelix containing a pseudoknot: histidinylation of turnip yellow mosaic virus RNA.

Authors:  J Rudinger; C Florentz; T Dreher; R Giegé
Journal:  Nucleic Acids Res       Date:  1992-04-25       Impact factor: 16.971

3.  Binding of tobramycin leads to conformational changes in yeast tRNA(Asp) and inhibition of aminoacylation.

Authors:  Frank Walter; Joern Pütz; Richard Giegé; Eric Westhof
Journal:  EMBO J       Date:  2002-02-15       Impact factor: 11.598

4.  Nucleotide sequence of the gene coding for yeast cytoplasmic aspartyl-tRNA synthetase (APS); mapping of the 5' and 3' termini of AspRS mRNA.

Authors:  M Sellami; F Fasiolo; G Dirheimer; J P Ebel; J Gangloff
Journal:  Nucleic Acids Res       Date:  1986-02-25       Impact factor: 16.971

5.  New photoactivatable structural and affinity probes of RNAs: specific features and applications for mapping of spermine binding sites in yeast tRNA(Asp) and interaction of this tRNA with yeast aspartyl-tRNA synthetase.

Authors:  A Garcia; R Giegé; J P Behr
Journal:  Nucleic Acids Res       Date:  1990-01-11       Impact factor: 16.971

6.  Influence of tRNA tertiary structure and stability on aminoacylation by yeast aspartyl-tRNA synthetase.

Authors:  J D Puglisi; J Pütz; C Florentz; R Giegé
Journal:  Nucleic Acids Res       Date:  1993-01-11       Impact factor: 16.971

7.  Determinant nucleotides of yeast tRNA(Asp) interact directly with aspartyl-tRNA synthetase.

Authors:  J Rudinger; J D Puglisi; J Pütz; D Schatz; F Eckstein; C Florentz; R Giegé
Journal:  Proc Natl Acad Sci U S A       Date:  1992-07-01       Impact factor: 11.205

8.  Aspartyl-tRNA synthetase from Escherichia coli: cloning and characterisation of the gene, homologies of its translated amino acid sequence with asparaginyl- and lysyl-tRNA synthetases.

Authors:  G Eriani; G Dirheimer; J Gangloff
Journal:  Nucleic Acids Res       Date:  1990-12-11       Impact factor: 16.971

9.  Antideterminants present in minihelix(Sec) hinder its recognition by prokaryotic elongation factor Tu.

Authors:  J Rudinger; R Hillenbrandt; M Sprinzl; R Giegé
Journal:  EMBO J       Date:  1996-02-01       Impact factor: 11.598

10.  Role of dimerization in yeast aspartyl-tRNA synthetase and importance of the class II invariant proline.

Authors:  G Eriani; J Cavarelli; F Martin; G Dirheimer; D Moras; J Gangloff
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-15       Impact factor: 11.205
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