Literature DB >> 6360251

Immunoelectron microscopy of the bone marrow mononuclears labelling with rabbit anti-mouse brain serum using peroxidase-anti-peroxidase method.

Z A Butenko, K P Zak, S V Komissarenko, M A Gruzov, B M Khomenko.   

Abstract

Ultrastructural features of mouse bone marrow mononuclears (BMM) labelled with peroxidase-anti-peroxidase (PAP) complex using rabbit anti-mouse brain serum (RAMBS) and rabbit anti-mouse brain serum, absorbed with thymocytes (RAMBST) were investigated. After incubation of cells with RAMBST which have high activity against mouse pluripotent hemopoietic stem cells (CFUs) about 0.6% of BMM were labelled. According to their fine structure these cells were similar to those described earlier as presumptive pluripotent hematopoietic stem cells (PHSC). After incubation with RAMBS 1.9% of BMM were labelled, including cells marked by RAMBST and typical small lymphocytes with ultrastructure of T-lymphocytes.

Entities:  

Mesh：

Year:  1983        PMID： 6360251     DOI： 10.1007/bf00320348

Source DB:  PubMed          Journal:  Blut        ISSN： 0006-5242


  17 in total

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Authors:  J E TILL; E A McCULLOCH
Journal:  Radiat Res       Date:  1961-02       Impact factor: 2.841

2.  Isolation of lymphocytes, granulocytes and macrophages.

Authors:  A Bøyum
Journal:  Scand J Immunol       Date:  1976-06       Impact factor: 3.487

3.  Brain-associated theta antigen: reactivity of rabbit anti-mouse brain with mouse lymphoid cells.

Authors:  E S Golub
Journal:  Cell Immunol       Date:  1971-08       Impact factor: 4.868

4.  Attempts at identification of hemopoietic stem cell in mouse.

Authors:  D W van Bekkum; M J van Noord; B Maat; K A Dicke
Journal:  Blood       Date:  1971-11       Impact factor: 22.113

5.  [Possibility of detecting hematopoietic stem cells by electron microscopy].

Authors:  R E Kavetskiĭ; Z A Butenko; K P Zak; E V Klochko; M A Baranovskiĭ
Journal:  Dokl Akad Nauk SSSR       Date:  1974

6.  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors:  R C Graham; M J Karnovsky
Journal:  J Histochem Cytochem       Date:  1966-04       Impact factor: 2.479

7.  Expression of Thy-1 antigen is not limited to T cells in cultures of mouse hemopoietic cells.

Authors:  J W Schrader; F Battye; R Scollay
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

8.  Direct identification of the murine pluripotential stem cell using rabbit anti-mouse brain serum.

Authors:  J A Filppi; J L Shellhaas; M S Rheins
Journal:  Transplantation       Date:  1978-06       Impact factor: 4.939

9.  Brain-associated stem cell antigen: an antigen shared by brain and hemopoietic stem cells.

Authors:  E S Golub
Journal:  J Exp Med       Date:  1972-08-01       Impact factor: 14.307

10.  Analysis of rat hemopoietic cells on the fluorescence-activated cell sorter. I. Isolation of pluripotent hemopoietic stem cells and granulocyte-macrophage progenitor cells.

Authors:  I Goldschneider; D Metcalf; F Battye; T Mandel
Journal:  J Exp Med       Date:  1980-08-01       Impact factor: 14.307
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