Literature DB >> 6358198

In vitro coupled transcription-translation of linear DNA fragments in a lysate derived from a recB rna pnp strain of Escherichia coli.

C L Bassett, J R Rawson.   

Abstract

A recB21 derivative (CLB7) of an Escherichia coli rna-19 pnp-7 strain (PR7) was constructed for use in examining the in vitro coupled transcription-translation of linear DNA. The expression of linearized DNAs in CLB7 (recB21 rna-19 pnp-7) lysates was enhanced significantly when compared with expression of the same DNAs in lysates prepared from the PR7 or the original recB21 (CF300) strains. In addition, the endogenous incorporation of [35S]methionine into protein was considerably reduced in CLB7 lysates relative to lysates derived from the original recB21 strain.

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Year:  1983        PMID： 6358198      PMCID： PMC217990          DOI： 10.1128/jb.156.3.1359-1362.1983

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  9 in total

1.  Increased expression of a eukaryotic gene in Escherichia coli through stabilization of its messenger RNA.

Authors:  J A Hautala; C L Bassett; N H Giles; S R Kushner
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Cell-free coupled transcription-translation system for investigation of linear DNA segments.

Authors:  H L Yang; L Ivashkiv; H Z Chen; G Zubay; M Cashel
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205

4.  Expression of cloned chloroplast DNA from Euglena gracilis in an in vitro DNA-dependent transcription-translation system prepared from E. coli.

Authors:  W H Andrews; J R Rawson
Journal:  Plasmid       Date:  1982-09       Impact factor: 3.466

5.  Physical and genetic characterization of the cloned sbcB (exonuclease I) region of the Escherichia coli genome.

Authors:  D Prasher; D A Kasunic; S R Kushner
Journal:  J Bacteriol       Date:  1983-02       Impact factor: 3.490

6.  DNA repair in Escherichia coli: identification of the uvrD gene product.

Authors:  V F Maples; S R Kushner
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

7.  Cell-free synthesis of proteins coding for mobilisation functions of ColE1 and transposition functions of Tn3.

Authors:  J Collins
Journal:  Gene       Date:  1979-05       Impact factor: 3.688

8.  Expression of the HIS3 gene of Saccharomyces cerevisiae in polynucleotide phosphorylase-deficient strains of Escherichia coli K-12.

Authors:  D A Kasunic; S R Kushner
Journal:  Gene       Date:  1980-12       Impact factor: 3.688

9.  Genetic analysis of recombination-deficient mutants of Escherichia coli K-12 carrying rec mutations cotransducible with thyA.

Authors:  N S Willetts; D W Mount
Journal:  J Bacteriol       Date:  1969-11       Impact factor: 3.490
  9 in total
  1 in total

1.  Decentralizing Cell-Free RNA Sensing With the Use of Low-Cost Cell Extracts.

Authors:  Anibal Arce; Fernando Guzman Chavez; Chiara Gandini; Juan Puig; Tamara Matute; Jim Haseloff; Neil Dalchau; Jenny Molloy; Keith Pardee; Fernán Federici
Journal:  Front Bioeng Biotechnol       Date:  2021-08-23
  1 in total

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