Literature DB >> 6358148

Quantitative estimation of pituitary gonadotropin in juvenile rainbow trout with scanning cytophotometry applied to immunocytochemical preparations.

M Terlou, R van den Hurk, J T Gielen.   

Abstract

A densitometrical method with computer-controlled scanning cytophotometry has been developed to quantify the amount of gonadotropin (GTH) in immunocytochemically treated paraffin sections of pituitaries of juvenile rainbow trout. Results obtained with this method totally correspond with those established with a radioimmunoassay of pooled pituitaries. Scanningcytophotometry offers the advantage that GTH-content of individual pituitaries of juvenile or small adult fish can be investigated.

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Year:  1983        PMID： 6358148     DOI： 10.1007/bf00489785

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  8 in total

1.  A useful technique applicable to the immunofluorescence test.

Authors:  K S Tung
Journal:  J Immunol Methods       Date:  1977       Impact factor: 2.303

2.  Densitometric measurement of two cellular parameters related to the secretory activity of the subcommissural organ.

Authors:  J H Diederen; H G Vullings; E J Van der Vlist; G G Legerstee-Oostveen
Journal:  Microsc Acta       Date:  1981-09

3.  Estradiol-positive feedback on gonadotropic (GTH) cells in freshwater male silver eels.

Authors:  M Olivereau; J Olivereau
Journal:  Gen Comp Endocrinol       Date:  1979-11       Impact factor: 2.822

4.  The brain-pituitary-gonadal axis in the rainbow trout, Salmo gairdneri. II. Direct effect of gonadal steroids on the gonadotropic cells.

Authors:  J T Gielen; H J Goos
Journal:  Cell Tissue Res       Date:  1983       Impact factor: 5.249

5.  The brain-pituitary-gonadal axis in the rainbow trout, Salmo gairdneri: gonadal hormones and the maturation of gonadotropic cells.

Authors:  J T Gielen; H J Goos; J Peute; R A van den Bosch; P G van Oordt
Journal:  Cell Tissue Res       Date:  1982       Impact factor: 5.249

6.  Accumulation of glycoprotein gonadotropin in the pituitary of juvenile rainbow trout in response to androgens and C21-steroids, including 11-steroids.

Authors:  R van den Hurk; J T Gielen; M Terlou
Journal:  Cell Tissue Res       Date:  1984       Impact factor: 5.249

7.  Effects of steroids on gonadotropic (GTH) cells in the pituitary of rainbow trout, Salmo gairdneri, shortly after hatching.

Authors:  R van den Hurk
Journal:  Cell Tissue Res       Date:  1982       Impact factor: 5.249

8.  Steroidogenesis in the gonads of rainbow trout fry (Salmo gairdneri) before and after the onset of gonadal sex differentiation.

Authors:  R van den Hurk; J G Lambert; J Peute
Journal:  Reprod Nutr Dev       Date:  1982
  8 in total
  1 in total

1.  Accumulation of glycoprotein gonadotropin in the pituitary of juvenile rainbow trout in response to androgens and C21-steroids, including 11-steroids.

Authors:  R van den Hurk; J T Gielen; M Terlou
Journal:  Cell Tissue Res       Date:  1984       Impact factor: 5.249
  1 in total

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