Literature DB >> 6356978

A one-step procedure for the purification of uridine phosphorylase from Escherichia coli.

A Vita, G Magni.   

Abstract

Adsorption to Matrex Gel Green A and successive elution with uridine provides a one-step procedure for the purification of uridine phosphorylase from Escherichia coli B. The homogeneous preparation of the enzyme can be obtained within a single day with an activity recovery above 80%.

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Year:  1983        PMID： 6356978     DOI： 10.1016/0003-2697(83)90236-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  2 in total

1.  Modification of uridine phosphorylase from Escherichia coli by diethyl pyrocarbonate. Evidence for a histidine residue in the active site of the enzyme.

Authors:  A K Drabikowska; G Woźniak
Journal:  Biochem J       Date:  1990-09-01       Impact factor: 3.857

2.  Nucleotide sequence of the Escherichia coli uridine phosphorylase (udp) gene.

Authors:  L Walton; C A Richards; L P Elwell
Journal:  Nucleic Acids Res       Date:  1989-08-25       Impact factor: 16.971
  2 in total

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