Literature DB >> 6356186

Cloning and deletion mapping of the recF dnaN region of the Escherichia coli chromosome.

L W Ream, A J Clark.   

Abstract

By cloning a 3.6-kb EcoRI fragment of the Escherichia coli chromosome with pBR322 we located more precisely recF relative to dnaN. By deletion mapping we localized functional recF to a 1.65-kb region of the cloned fragment and allowed rough mapping of the C terminus of dnaN. Cloned recF+, separated from functional flanking genes dnaN and gyrB, complemented chromosomal recF mutations presumably by coding for a cytodiffusible product. The protein encoded by dnaN was observed as a band on a polyacrylamide gel from minicells. Identification of a recF protein was not made.

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Year:  1983        PMID： 6356186     DOI： 10.1016/0147-619x(83)90062-8

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  10 in total

1.  Role of Escherichia coli DNA polymerase I in conferring viability upon the dnaN159 mutant strain.

Authors:  Robert W Maul; Laurie H Sanders; James B Lim; Rosemary Benitez; Mark D Sutton
Journal:  J Bacteriol       Date:  2007-04-20       Impact factor: 3.490

Review 2.  Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Authors:  M K Berlyn
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

Review 3.  Linkage map of Escherichia coli K-12, edition 8.

Authors:  B J Bachmann
Journal:  Microbiol Rev       Date:  1990-06

4.  Cloning of the Escherichia coli recJ chromosomal region and identification of its encoded proteins.

Authors:  S T Lovett; A J Clark
Journal:  J Bacteriol       Date:  1985-04       Impact factor: 3.490

5.  Mutation-dependent suppression of recB21 recC22 by a region cloned from the Rac prophage of Escherichia coli K-12.

Authors:  D K Willis; L H Satin; A J Clark
Journal:  J Bacteriol       Date:  1985-06       Impact factor: 3.490

6.  DNA sequence and transcription of the region upstream of the E. coli gyrB gene.

Authors:  T Adachi; K Mizuuchi; R Menzel; M Gellert
Journal:  Nucleic Acids Res       Date:  1984-08-24       Impact factor: 16.971

7.  Heterogeneity in the level of ampicillin resistance conferred by pBR322 derivatives with different DNA supercoiling.

Authors:  V Aleixandre; M Blanco
Journal:  Mol Gen Genet       Date:  1987-08

8.  Two-component suppression of recF143 by recA441 in Escherichia coli K-12.

Authors:  M R Volkert; L J Margossian; A J Clark
Journal:  J Bacteriol       Date:  1984-11       Impact factor: 3.490

9.  Molecular analysis of the recF gene of Escherichia coli.

Authors:  M A Blanar; S J Sandler; M E Armengod; L W Ream; A J Clark
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205

10.  Sequence and complementation analysis of recF genes from Escherichia coli, Salmonella typhimurium, Pseudomonas putida and Bacillus subtilis: evidence for an essential phosphate binding loop.

Authors:  S J Sandler; B Chackerian; J T Li; A J Clark
Journal:  Nucleic Acids Res       Date:  1992-02-25       Impact factor: 16.971
  10 in total

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