Literature DB >> 6355770

Marker rescue with plasmids bearing deletions in rpoD identifies a dispensable part of E. coli sigma factor.

J C Hu, C A Gross.   

Abstract

Recombinant DNA techniques allow rapid construction and characterization of tools for genetic dissection of essential genes. In this report, we describe construction of a set of deletions in rpoD, the gene which encodes the sigma subunit of E. coli RNA polymerase. We used this deletion set to identify a region of the sigma polypeptide which is dispensable for its function.

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Year:  1983        PMID： 6355770     DOI： 10.1007/bf00425768

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  25 in total

1.  The differential precipition of nucleic acids and proteins from aqueous solutions by ethanol.

Authors:  J Wilcockson
Journal:  Anal Biochem       Date:  1975-05-26       Impact factor: 3.365

2.  Genetic studies of the lac repressor. VII. On the molecular nature of spontaneous hotspots in the lacI gene of Escherichia coli.

Authors:  P J Farabaugh; U Schmeissner; M Hofer; J H Miller
Journal:  J Mol Biol       Date:  1978-12-25       Impact factor: 5.469

3.  Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o.

Authors:  H M Goodman; M V Olson; B D Hall
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

4.  The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12.

Authors:  Z F Burton; C A Gross; K K Watanabe; R R Burgess
Journal:  Cell       Date:  1983-02       Impact factor: 41.582

5.  Mutations in the Ion gene of E. coli K12 phenotypically suppress a mutation in the sigma subunit of RNA polymerase.

Authors:  A D Grossman; R R Burgess; W Walter; C A Gross
Journal:  Cell       Date:  1983-01       Impact factor: 41.582

6.  A rapid boiling method for the preparation of bacterial plasmids.

Authors:  D S Holmes; M Quigley
Journal:  Anal Biochem       Date:  1981-06       Impact factor: 3.365

7.  Cascades of Sigma factors.

Authors:  R Losick; J Pero
Journal:  Cell       Date:  1981-09       Impact factor: 41.582

8.  Mutation affecting thermostability of sigma subunit of Escherichia coli RNA polymerase lies near the dnaG locus at about 66 min on the E. coli genetic map.

Authors:  C Gross; J Hoffman; C Ward; D Hager; G Burdick; H Berger; R Burgess
Journal:  Proc Natl Acad Sci U S A       Date:  1978-01       Impact factor: 11.205

9.  Temperature-sensitive Escherichia coli mutant producing a temperature-sensitive sigma subunit of DNA-dependent RNA polymerase.

Authors:  J D Harris; J S Heilig; I I Martinez; R Calendar; L A Isaksson
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

10.  Isolation and characterization of transducing phage coding for sigma subunit of Escherichia coli RNA polymerase.

Authors:  C A Gross; F R Blattner; W E Taylor; P A Lowe; R R Burgess
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205
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  17 in total

1.  How a mutation in the gene encoding sigma 70 suppresses the defective heat shock response caused by a mutation in the gene encoding sigma 32.

Authors:  Y N Zhou; C A Gross
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

2.  A mutant sigma 32 with a small deletion in conserved region 3 of sigma has reduced affinity for core RNA polymerase.

Authors:  Y N Zhou; W A Walter; C A Gross
Journal:  J Bacteriol       Date:  1992-08       Impact factor: 3.490

3.  Enhanced heterologous gene expression in novel rpoH mutants of Escherichia coli.

Authors:  M G Obukowicz; N R Staten; G G Krivi
Journal:  Appl Environ Microbiol       Date:  1992-05       Impact factor: 4.792

4.  Dominant lethal phenotype of a mutation in the -35 recognition region of Escherichia coli sigma 70.

Authors:  J Keener; M Nomura
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-01       Impact factor: 11.205

5.  Multiple regions on the Escherichia coli heat shock transcription factor sigma32 determine core RNA polymerase binding specificity.

Authors:  D M Joo; A Nolte; R Calendar; Y N Zhou; D J Jin
Journal:  J Bacteriol       Date:  1998-03       Impact factor: 3.490

6.  RNA polymerase beta mutations have reduced sigma70 synthesis leading to a hyper-temperature-sensitive phenotype of a sigma70 mutant.

Authors:  Y N Zhou; D J Jin
Journal:  J Bacteriol       Date:  1997-07       Impact factor: 3.490

Review 7.  Multiple procaryotic ribonucleic acid polymerase sigma factors.

Authors:  R H Doi; L F Wang
Journal:  Microbiol Rev       Date:  1986-09

8.  Sigma factors from E. coli, B. subtilis, phage SP01, and phage T4 are homologous proteins.

Authors:  M Gribskov; R R Burgess
Journal:  Nucleic Acids Res       Date:  1986-08-26       Impact factor: 16.971

9.  Deletion and insertion mutations in the rpoH gene of Escherichia coli that produce functional sigma 32.

Authors:  R Calendar; J W Erickson; C Halling; A Nolte
Journal:  J Bacteriol       Date:  1988-08       Impact factor: 3.490

10.  Regulation of cyclic AMP synthesis in Escherichia coli K-12: effects of the rpoD800 sigma mutation, glucose, and chloramphenicol.

Authors:  A D Grossman; A Ullmann; R R Burgess; C A Gross
Journal:  J Bacteriol       Date:  1984-04       Impact factor: 3.490
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