Literature DB >> 6355168

Rapid immunoperoxidase assay for detection of respiratory syncytial virus in nasopharyngeal secretions.

R Cevenini, M Donati, A Moroni, L Franchi, F Rumpianesi.   

Abstract

Samples of nasopharyngeal secretions obtained from 70 infants and young children with acute respiratory disease were examined for the presence of respiratory syncytial virus by immunoperoxidase assay (IPA). The IPA was compared with the immunofluorescence assay and with cell culture isolation. Respiratory syncytial virus antigen-positive cells were detected by both IPA and immunofluorescence assay in 28 specimens; 25 samples were positive in cell culture. The agreement between virus isolation and IPA and IFA was 89%. The applicability of IPA to rapid viral diagnosis of respiratory syncytial virus infection is discussed.

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Year:  1983        PMID： 6355168      PMCID： PMC270936          DOI： 10.1128/jcm.18.4.947-949.1983

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

1.  A rapid immunoperoxidase assay for the detection of specific IgG antibodies to Chlamydia trachomatis.

Authors:  R Cevenini; F Rumpianesi; M Donati; I Sarov
Journal:  J Clin Pathol       Date:  1983-03       Impact factor: 3.411

2.  Immunoperoxidase antibody to human cytomegalovirus-induced membrane antigen assay in the absence of interfering immunoglobulin G receptors.

Authors:  H Haikin; I Sarov
Journal:  Intervirology       Date:  1980       Impact factor: 1.763

3.  Detection of virus-specific IgA antibodies in serum of kidney transplant patients with recurrent cytomegalovirus infection by enzymeimmuno and radioimmunoassay techniques.

Authors:  I Sarov; E Levy; M Aymard; Y Chardonnet; S Bosshard; J P Revillard; M Friedman; E Nord; M Greiff; H Haikin
Journal:  Clin Exp Immunol       Date:  1982-05       Impact factor: 4.330

4.  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors:  R C Graham; M J Karnovsky
Journal:  J Histochem Cytochem       Date:  1966-04       Impact factor: 2.479

5.  Antibody to varicella-zoster virus-induced membrane antigen: immunoperoxidase assay with air-dried target cells.

Authors:  H Haikin; S Leventon-Kriss; I Sarov
Journal:  J Infect Dis       Date:  1979-10       Impact factor: 5.226

6.  Detection of respiratory syncytial virus in nasal secretions from infants by enzyme-linked immunosorbent assay.

Authors:  R K Chao; M Fishaut; J D Schwartzman; K McIntosh
Journal:  J Infect Dis       Date:  1979-04       Impact factor: 5.226

7.  Comparison of immunofluorescence and immunoperoxidase methods for viral diagnosis at a distance: a WHO collaborative study.

Authors:  P S Gardner; M Grandien; J McQuillin
Journal:  Bull World Health Organ       Date:  1978       Impact factor: 9.408

8.  Detection of respiratory syncytial, parainfluenza type 2, and adenovirus antigens by radioimmunoassay and enzyme immunoassay on nasopharyngeal specimens from children with acute respiratory disease.

Authors:  H K Sarkkinen; P E Halonen; P P Arstila; A A Salmi
Journal:  J Clin Microbiol       Date:  1981-02       Impact factor: 5.948

9.  Detection of respiratory syncytial virus in nasopharyngeal secretions by inhibition of enzyme-linked immunosorbent assay.

Authors:  A Hornsleth; E Brenøe; B Friis; F U Knudsen; P Uldall
Journal:  J Clin Microbiol       Date:  1981-11       Impact factor: 5.948

10.  The late detection of respiratory syncytial virus in cells of respiratory tract by immunofluorescence.

Authors:  P S Gardner; J McQuillin; R McGuckin
Journal:  J Hyg (Lond)       Date:  1970-12
  10 in total
  6 in total

1.  Detection of Semliki Forest virus in cell culture by use of an enzyme immunoassay with peroxidase-labeled monoclonal antibodies specific for glycoproteins E1 and E2.

Authors:  F H van Tiel; W A Boere; J Vinjé; T Harmsen; B J Benaissa-Trouw; C A Kraaijeveld; H Snippe
Journal:  J Clin Microbiol       Date:  1984-09       Impact factor: 5.948

2.  An immunoperoxidase assay for the detection of specific IgA antibody in Epstein-Barr virus infections.

Authors:  R Cevenini; M Donati; F Rumpianesi; A Moroni; P Paolucci
Journal:  J Clin Pathol       Date:  1984-04       Impact factor: 3.411

3.  Peroxidase-antiperoxidase assay for rapid detection of respiratory syncytial virus in nasal epithelial specimens from infants and children.

Authors:  A A Jalowayski; B L England; C J Temm; T J Nunemacher; J F Bastian; G A MacPherson; W M Dankner; R C Straube; J D Connor
Journal:  J Clin Microbiol       Date:  1987-04       Impact factor: 5.948

4.  Capture-ELISA for serum IgM antibody to respiratory syncytial virus.

Authors:  R Cevenini; M Donati; S Bertini; A Moroni; V Sambri
Journal:  J Hyg (Lond)       Date:  1986-12

5.  Detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay.

Authors:  J H Hughes; D R Mann; V V Hamparian
Journal:  J Clin Microbiol       Date:  1988-03       Impact factor: 5.948

6.  Application of immunoassay of encephalomyocarditis virus in cell culture with enzyme-labeled virus-specific monoclonal antibodies for rapid detection of virus, neutralizing antibodies, and interferon.

Authors:  F Vlaspolder; T Harmsen; D van Veenendaal; C A Kraaijeveld; H Snippe
Journal:  J Clin Microbiol       Date:  1988-12       Impact factor: 5.948
  6 in total

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