Literature DB >> 6355067

Procedure for mutagenizing spores of Saccharomyces cerevisiae.

P Romano, M G Soli, G Suzzi.   

Abstract

A procedure for inducing mutants of a homothallic strain of Saccharomyces cerevisiae is described. The essential parts of the procedure are long incubation in Glusulase, which preferentially kills vegetative cells instead of spores, and treatment in 9% ethyl methanesulfonate, which also preferentially kills vegetative cells instead of spores. Consequently, the viable population is virtually 100% spores.

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Year:  1983        PMID： 6355067      PMCID： PMC217912          DOI： 10.1128/jb.156.2.907-908.1983

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  5 in total

Review 1.  Preparation and growth of yeast protoplasts.

Authors:  S C Kuo; S Yamamoto
Journal:  Methods Cell Biol       Date:  1975       Impact factor: 1.441

2.  Use of snail digestive juice in isolation of yeast spore tetrads.

Authors:  J R JOHNSTON; R K MORTIMER
Journal:  J Bacteriol       Date:  1959-08       Impact factor: 3.490

3.  Preparation and storage of single spores of Saccharomyces cerevisiae.

Authors:  P Rousseau; H O Halvorson
Journal:  J Bacteriol       Date:  1969-12       Impact factor: 3.490

4.  Genetical mutants induced by ethyl methanesulfonate in Saccharomyces.

Authors:  G Lindegren; Y L Hwang; Y Oshima; C C Lindegren
Journal:  Can J Genet Cytol       Date:  1965-09

5.  An enrichment method for auxotrophic yeast mutants using the antibiotic 'nystatin'.

Authors:  R Snow
Journal:  Nature       Date:  1966-07-09       Impact factor: 49.962
  5 in total
  1 in total

Review 1.  Improving industrial yeast strains: exploiting natural and artificial diversity.

Authors:  Jan Steensels; Tim Snoek; Esther Meersman; Martina Picca Nicolino; Karin Voordeckers; Kevin J Verstrepen
Journal:  FEMS Microbiol Rev       Date:  2014-05-08       Impact factor: 16.408
  1 in total

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