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Literature DB >> 6354508

Further characterization of filarial antigens by SDS polyacrylamide gel electrophoresis.

S Dissanayake, S C Galahitiyawa, M M Ismail.

Abstract

SDS (sodium dodecyl sulfate)-polyacrylamide gel electrophoresis of an antigen isolated from sera of Wuchereria bancrofti-infected patients and Setaria digitata antigen SD2-4 is reported. Both antigens showed carbohydrate (glycoprotein) staining. The W. bancrofti antigen had an apparent relative molecular mass of 35 000 while the S. digitata antigen SD2-4 migrated at the marker dye position on SDS-polyacrylamide gel electrophoresis. SDS treatment of these antigens did not abolish the precipitation reaction with antibody. In the case of W. bancrofti antigen, SDS treatment probably exposed hitherto hidden antigen epitopes.

Entities: Chemical Disease Species

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Year: 1983 PMID： 6354508 PMCID： PMC2536150

Source DB: PubMed Journal: Bull World Health Organ ISSN： 0042-9686 Impact factor: 9.408

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References

18 in total

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Journal: Am J Trop Med Hyg Date: 1981-03 Impact factor: 2.345

Further characterization of filarial antigens by SDS polyacrylamide gel electrophoresis.

1. Preparation of iodine-131 labelled human growth hormone of high specific activity.

2. Studies on hemagglutination test on filariasis. I. The fractionation and purification of antigens by column chromatography and disc electrophoresis.

3. The binding of detergents to proteins. I. The maximum amount of dodecyl sulfate bound to proteins and the resistance to binding of several proteins.

4. Studies on skin test antigen FST for immunodiagnosis of filariasis. 3. Separation and characterization of FST3-1 by isoelectric focusing technique.

5. Studies on skin test antigen FST for immunodiagnosis of filariasis. I. Electrophoretic analysis and fractionation of antigen FST.

6. Immune complexes in Wuchereria bancrofti infection in man.

7. Circulating immune complexes in experimental filariasis.

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9. Antibody determination in the diagnosis of Wuchereria bancrofti infection in man.

10. Antibody response to heterologous and homologous antigens in Brugia malayi- and B. pahangi-infected Mongolian jirds as measured by the enzyme-linked immunosorbent assay (ELISA).