Literature DB >> 6354183

A rapid procedure for the preparation of highly purified pyruvate decarboxylase from brewer's yeast.

M Sieber, S König, G Hübner, A Schellenberger.   

Abstract

A rapid purification procedure for pyruvate decarboxylase (E.C. 4.1.1.1.) from fresh cells of brewer's yeast (Saccharomyces carlsb.) is reported. The preparation of a crude enzyme (30-45 U/mg) by the use of fractionation steps with protamine sulfate, acetone, and ammonium sulfate takes about 6-7 h. A stable pyruvate decarboxylase (70-85 U/mg) was obtained from such preparations after purification on CM Sephadex C 50 after another 2-3 h. Stability and structural properties are compared for enzymes prepared from fresh and dried yeast.

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Year:  1983        PMID： 6354183

Source DB:  PubMed          Journal:  Biomed Biochim Acta        ISSN： 0232-766X


  5 in total

1.  Pyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon, Pyrococcus furiosus, functions as a CoA-dependent pyruvate decarboxylase.

Authors:  K Ma; A Hutchins; S J Sung; M W Adams
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-02       Impact factor: 11.205

2.  pdc1(0) mutants of Saccharomyces cerevisiae give evidence for an additional structural PDC gene: cloning of PDC5, a gene homologous to PDC1.

Authors:  P G Seeboth; K Bohnsack; C P Hollenberg
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

3.  Pyruvate decarboxylase from Zymomonas mobilis. Structure and re-activation of apoenzyme by the cofactors thiamin diphosphate and magnesium ion.

Authors:  R J Diefenbach; R G Duggleby
Journal:  Biochem J       Date:  1991-06-01       Impact factor: 3.857

4.  The influence of the effectors of yeast pyruvate decarboxylase (PDC) on the conformation of the dimers and tetramers and their pH-dependent equilibrium.

Authors:  S König; D Svergun; M H Koch; G Hübner; A Schellenberger
Journal:  Eur Biophys J       Date:  1993       Impact factor: 1.733

5.  The influence of protein concentration on oligomer structure and catalytic function of two pyruvate decarboxylases.

Authors:  Steffen Kutter; Michael Spinka; Michel H J Koch; Stephan König
Journal:  Protein J       Date:  2007-12       Impact factor: 2.371
  5 in total

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