Literature DB >> 6352798

A method for examining the endothelial cytoskeleton in situ using immunofluorescence.

K A Rogers, V I Kalnins.   

Abstract

A simple technique is described for producing en face preparations of endothelial cells (EC) from large blood vessels fixed in situ that are suitable for studying the distribution of specific antigens in EC by immunofluorescence. This method has permitted us to examine the distribution of various components of the cytoskeleton, including microtubules (MT), centrioles, and microfilaments (MF) in EC in vivo.

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Year:  1983        PMID： 6352798     DOI： 10.1177/31.11.6352798

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  5 in total

1.  Role of actin and myosin in the control of paracellular permeability in pig, rat and human vascular endothelium.

Authors:  H J Schnittler; A Wilke; T Gress; N Suttorp; D Drenckhahn
Journal:  J Physiol       Date:  1990-12       Impact factor: 5.182

2.  The distribution of centrosomes in endothelial cells of non-wounded and wounded aortic organ cultures.

Authors:  K A Rogers; P Boden; V I Kalnins; A I Gotlieb
Journal:  Cell Tissue Res       Date:  1986       Impact factor: 5.249

3.  Postnatal reorganization of actin filaments and differentiation of intercellular boundaries in the rat aortic endothelial cells.

Authors:  N Kobayashi; T Sakai
Journal:  Cell Tissue Res       Date:  1994-12       Impact factor: 5.249

4.  Preferential orientation of centrioles toward the heart in endothelial cells of major blood vessels is reestablished after reversal of a segment.

Authors:  K A Rogers; N H McKee; V I Kalnins
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

5.  En Face Preparation of Mouse Blood Vessels.

Authors:  Kyung Ae Ko; Keigi Fujiwara; Sunil Krishnan; Jun-Ichi Abe
Journal:  J Vis Exp       Date:  2017-05-19       Impact factor: 1.355
  5 in total

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