Literature DB >> 6352294

A new method for the three-dimensional in vitro growth of human cancer cells.

H Z Kupchik, R S Langer, C Haberern, S El Deriny, M O'Brien.   

Abstract

A method has been developed which allows, for the first time, the in vitro growth of human tumor cells in calcium alginate gel microcapsules. The three-dimensional matrix is permeable to macromolecules of at least 200 000 molecular weight (MW) (e.g., carcinoembryonic antigen) and provides an environment for organized growth and differentiation more comparable to in vivo growth than monolayer culture. This inexpensive, nondestructive procedure allows studies of morphological, biochemical and immunochemical parameters of human tumor cells within a single model.

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Year:  1983        PMID： 6352294     DOI： 10.1016/0014-4827(83)90228-8

Source DB:  PubMed          Journal:  Exp Cell Res        ISSN： 0014-4827            Impact factor:   3.905


  5 in total

1.  A three-dimensional system for long-term culture of human colorectal adenomas.

Authors:  J C O'Keane; H Z Kupchik; P C Schroy; C D Andry; E Collins; M J O'Brien
Journal:  Am J Pathol       Date:  1990-12       Impact factor: 4.307

2.  Quantitative and qualitative differences in growth, invasion and lung colonization of an anaplastic and a papillary human thyroid cancer cell line in vitro and in vivo.

Authors:  E R Boghaert; K Ain; K Taylor; V L Greenberg; C Fowler; S G Zimmer
Journal:  Clin Exp Metastasis       Date:  1996-10       Impact factor: 5.150

3.  The influence of the presence of adenovirus 5 E1a and E1b sequences on the pathology of rat embryonic fibroblasts transfected with activated c-Ha-ras and v-ras.

Authors:  E R Boghaert; V Austin; S G Zimmer
Journal:  Clin Exp Metastasis       Date:  1991 May-Jun       Impact factor: 5.150

4.  Culture of chondrocytes in alginate gel: variations in conditions of gelation influence the structure of the alginate gel, and the arrangement and morphology of proliferating chondrocytes.

Authors:  M B Aydelotte; E J Thonar; J Mollenhauer; J Flechtenmacher
Journal:  In Vitro Cell Dev Biol Anim       Date:  1998-02       Impact factor: 2.416

5.  3D viability imaging of tumor phantoms treated with single-walled carbon nanohorns and photothermal therapy.

Authors:  Jon Whitney; Matthew DeWitt; Bryce M Whited; William Carswell; Alex Simon; Christopher G Rylander; Marissa Nichole Rylander
Journal:  Nanotechnology       Date:  2013-06-18       Impact factor: 3.874
  5 in total

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