Literature DB >> 6351845

Purification and partial characterization of a rat retina alcohol dehydrogenase active with ethanol and retinol.

P Julià, J Farrés, X Parés.   

Abstract

Homogeneous alcohol dehydrogenase (ADH) from rat retina was obtained by chromatography on DEAE-Sepharose and AMP-hexane-Sepharose. The enzyme is a dimer of Mr congruent to 80000 and oxidizes ethanol using NAD+ as a cofactor. Careful activity determinations demonstrate unambiguously that rat retina ADH is active with retinol as a substrate. This result opens the question about the role of retina ADH in the visual cycle.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6351845      PMCID： PMC1152161          DOI： 10.1042/bj2130547

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Human liver alcohol dehydrogenase: purification, composition, and catalytic features.

Authors:  L G Lange; A J Sytkowski; B L Vallee
Journal:  Biochemistry       Date:  1976-10-19       Impact factor: 3.162

3.  Studies on the properties of retinal alcohol dehydrogenase from the rat.

Authors:  W D Watkins; T R Tephly
Journal:  J Neurochem       Date:  1971-12       Impact factor: 5.372

4.  Enzymes catalysing ethanol metabolism in neural and somatic tissues of the rat.

Authors:  N H Raskin; L Sokoloff
Journal:  J Neurochem       Date:  1972-02       Impact factor: 5.372

5.  Pyrazole inhibition and kinetic studies of ethanol and retinol oxidation catalyzed by rat liver alcohol dehydrogenase.

Authors:  M Reynier
Journal:  Acta Chem Scand       Date:  1969

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Molar absorbance of cattle rhodopsin.

Authors:  F J Daemen; J M Borggreven; S L Bonting
Journal:  Nature       Date:  1970-09-19       Impact factor: 49.962

8.  Uptake of tritiated retinaldehyde by the visual pigment of dark adapted rats.

Authors:  C D Bridges; S Yoshikami
Journal:  Nature       Date:  1969-01-18       Impact factor: 49.962

9.  Retinol and alcohol dehydrogenases in retina and liver.

Authors:  A L Koen; C R Shaw
Journal:  Biochim Biophys Acta       Date:  1966-10-17

10.  Biochemical aspects of the visual process. XXVII. Stereospecificity of ocular retinol dehydrogenases and the visual cycle.

Authors:  F Lion; J P Rotmans; F J Daemen; S L Bonting
Journal:  Biochim Biophys Acta       Date:  1975-04-19
View more
  3 in total

1.  Biochemical properties of purified human retinol dehydrogenase 12 (RDH12): catalytic efficiency toward retinoids and C9 aldehydes and effects of cellular retinol-binding protein type I (CRBPI) and cellular retinaldehyde-binding protein (CRALBP) on the oxidation and reduction of retinoids.

Authors:  Olga V Belyaeva; Olga V Korkina; Anton V Stetsenko; Tom Kim; Peter S Nelson; Natalia Y Kedishvili
Journal:  Biochemistry       Date:  2005-05-10       Impact factor: 3.162

Review 2.  Medium- and short-chain dehydrogenase/reductase gene and protein families : Medium-chain and short-chain dehydrogenases/reductases in retinoid metabolism.

Authors:  X Parés; J Farrés; N Kedishvili; G Duester
Journal:  Cell Mol Life Sci       Date:  2008-12       Impact factor: 9.261

3.  Genetics of ocular NAD+-dependent alcohol dehydrogenase and aldehyde dehydrogenase in the mouse: evidence for genetic identity with stomach isozymes and localization of Ahd-4 on chromosome 11 near trembler.

Authors:  R S Holmes; R A Popp; J L VandeBerg
Journal:  Biochem Genet       Date:  1988-04       Impact factor: 1.890
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.