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Literature DB >> 6351841

Purification of the major endoglucanase from Aspergillus fumigatus Fresenius.

Abstract

Aspergillus fumigatus (Fresenius), IMI 246651, A.T.C.C. 46324, produces two beta-glucosidase enzymes, cotton-solubilizing activity, xylanase and endoglucanase enzymes which can be separated by gel-filtration chromatography. The major endoglucanase does not bind to concanavalin A-Sepharose and does not stain with periodic acid/Schiff reagent. It is homogeneous on polyacrylamide isoelectric focusing (pI = 7.1) and has a mol.wt. of 12500 by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The endoglucanase produces glucose and a mixture of oligosaccharides from cellulose; the purified enzyme has a small dextranase activity. It is stable at 50 degrees C and pH 6.

Entities: Chemical Species

Mesh：

Substances：
Cellulase

Year: 1983 PMID： 6351841 PMCID： PMC1152145 DOI： 10.1042/bj2130437

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

17 in total

1. The biological degradation of soluble cellulose derivatives and its relationship to the mechanism of cellulose hydrolysis.

Authors: E T REESE; R G H SIU; H S LEVINSON
Journal: J Bacteriol Date: 1950-04 Impact factor: 3.490

2. Purification and properties of an endo-cellulase of avicelase type from Irpex lacteus (Polyporus tulipiferae).

Authors: T Kanda; K Wakabayashi; K Nisizawa
Journal: J Biochem Date: 1976-05 Impact factor: 3.387

3. The mechanism of enzymatic cellulose degradation. Purification of a cellulolytic enzyme from Trichoderma viride active on highly ordered cellulose.

Authors: L E Berghem; L G Pettersson
Journal: Eur J Biochem Date: 1973-08-01

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. The gel-filtration behaviour of proteins related to their molecular weights over a wide range.

Authors: P Andrews
Journal: Biochem J Date: 1965-09 Impact factor: 3.857

6. Extracellular enzyme system utilized by the fungus Sporotrichum pulverulentum (Chrysosporium lignorum) for the breakdown of cullulose. Functional characterization of five endo-1,4-beta-glucanases and one exo-1,4-beta-glucanase.

Authors: M Streamer; K E Eriksson; B Pettersson
Journal: Eur J Biochem Date: 1975-11-15

4. Endoglucanase gene of M42 aminopeptidase/endoglucanase family from thermophilic Bacillus sp. PW1 and PW2 isolated from Tattapani hot spring, Himachal Pradesh, India.

Authors: Divyanshi Sharma; Parul Sharma; Kamal Dev; Anuradha Sourirajan
Journal: J Genet Eng Biotechnol Date: 2019-10-02

4 in total

Purification of the major endoglucanase from Aspergillus fumigatus Fresenius.

1. The biological degradation of soluble cellulose derivatives and its relationship to the mechanism of cellulose hydrolysis.

2. Purification and properties of an endo-cellulase of avicelase type from Irpex lacteus (Polyporus tulipiferae).

3. The mechanism of enzymatic cellulose degradation. Purification of a cellulolytic enzyme from Trichoderma viride active on highly ordered cellulose.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. The gel-filtration behaviour of proteins related to their molecular weights over a wide range.

6. Extracellular enzyme system utilized by the fungus Sporotrichum pulverulentum (Chrysosporium lignorum) for the breakdown of cullulose. Functional characterization of five endo-1,4-beta-glucanases and one exo-1,4-beta-glucanase.

7. The purification and properties of the C 1 component of Trichoderma koningii cellulase.

8. The cellulase of Trichoderma viride. Separation of the components involved in the solubilization of cotton.

9. Factors influencing the production of cellulase by Aspergillus fumigatus (Fresenius).

10. Estimation of the molecular weights of proteins by Sephadex gel-filtration.

Review 1. Aspergillus enzymes involved in degradation of plant cell wall polysaccharides.

2. Purification and characterization of an acidothermophilic cellulase enzyme produced by Bacillus subtilis strain LFS3.

3. Purification, Characterization, and Application of Endoglucanase from Rhizopus oryzae as Antibiofilm Agent.

4. Endoglucanase gene of M42 aminopeptidase/endoglucanase family from thermophilic Bacillus sp. PW1 and PW2 isolated from Tattapani hot spring, Himachal Pradesh, India.