Literature DB >> 6348163

Anti-glomerular basement membrane autoantibodies in the Brown Norway rat: detection by a solid-phase radioimmunoassay.

C Bowman, D K Peters, C M Lockwood.   

Abstract

A solid-phase radioimmunoassay (RIA) is described for the detection of IgG autoantibodies to glomerular basement membrane (GBM) induced in the Brown Norway rat by mercuric chloride. The assay involves the adsorption of a collagenase digest of GBM to plastic microtitre plates and detection of bound antibody with affinity purified radiolabelled rabbit anti-rat IgG. Comparison with existing immunofluorescence methods for detection of anti-GBM antibody showed that the solid-phase RIA is highly sensitive, allowing detection of antibody in solutions with as low as 0.5 ng protein/ml. The assay is suitable for detection of anti-GBM antibody both in serum and in eluates from nephritic kidneys. The assay proved to be specific in competitive studies of inhibition brought by GBM, keyhole limpet antigen and ovalbumin. This solid-phase RIA is reproducible, robust and easy to perform.

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Year:  1983        PMID： 6348163     DOI： 10.1016/0022-1759(83)90227-2

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  14 in total

1.  Cyclosporin A in the prevention and treatment of experimental autoimmune glomerulonephritis in the brown Norway rat.

Authors:  J Reynolds; S J Cashman; D J Evans; C D Pusey
Journal:  Clin Exp Immunol       Date:  1991-07       Impact factor: 4.330

2.  Kinetics and pathogenicity of autoantibodies induced by mercuric chloride in the brown Norway rat.

Authors:  C D Pusey; C Bowman; A Morgan; A P Weetman; B Hartley; C M Lockwood
Journal:  Clin Exp Immunol       Date:  1990-07       Impact factor: 4.330

3.  Detection of Goodpasture antigen in fractions prepared from collagenase digests of human glomerular basement membrane.

Authors:  A J Fish; M C Lockwood; M Wong; R G Price
Journal:  Clin Exp Immunol       Date:  1984-01       Impact factor: 4.330

4.  Mercuric chloride-induced autoimmunity in the brown Norway rat. Cellular kinetics and major histocompatibility complex antigen expression.

Authors:  J Aten; C B Bosman; J Rozing; T Stijnen; P J Hoedemaeker; J J Weening
Journal:  Am J Pathol       Date:  1988-10       Impact factor: 4.307

5.  Cyclosporin A induces long-term unresponsiveness in mercuric chloride-induced autoimmune glomerulonephritis.

Authors:  J Aten; C B Bosman; E De Heer; P J Hoedemaeker; J J Weening
Journal:  Clin Exp Immunol       Date:  1988-08       Impact factor: 4.330

6.  Recombinant alpha-chains of type IV collagen demonstrate that the amino terminal of the Goodpasture autoantigen is crucial for antibody recognition.

Authors:  J J Ryan; P J Mason; C D Pusey; N Turner
Journal:  Clin Exp Immunol       Date:  1998-07       Impact factor: 4.330

7.  Effects of cyclophosphamide on autoantibody synthesis in the Brown Norway rat.

Authors:  C D Pusey; C Bowman; D K Peters; C M Lockwood
Journal:  Clin Exp Immunol       Date:  1983-12       Impact factor: 4.330

8.  In vivo treatment with a monoclonal antibody to T helper cells in experimental autoimmune glomerulonephritis in the BN rat.

Authors:  J Reynolds; C D Pusey
Journal:  Clin Exp Immunol       Date:  1994-01       Impact factor: 4.330

9.  Circulating T-cell populations during mercuric chloride-induced nephritis in the Brown Norway rat.

Authors:  C Bowman; C Green; L Borysiewicz; C M Lockwood
Journal:  Immunology       Date:  1987-08       Impact factor: 7.397

10.  Pathogenesis of experimental lupus nephritis: a role for anti-basement membrane and anti-tubular brush border antibodies in murine chronic graft-versus-host disease.

Authors:  J A Bruijn; P C Hogendoorn; W E Corver; L J van den Broek; P J Hoedemaeker; G J Fleuren
Journal:  Clin Exp Immunol       Date:  1990-01       Impact factor: 4.330
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