Literature DB >> 6347893

Immunochemical characterization of Aspergillus fumigatus antigens.

V P Kurup, E Y Ting, J N Fink.   

Abstract

Culture filtrate antigens of Aspergillus fumigatus Ag 534 were purified by preparative isoelectric focusing and affinity chromatography. One of the pooled antigen fractions from the preparative isoelectric focusing step (pool 2) was passed through a concanavalin A column and yielded two components, designated antigens IIa and IIb. Antigen IIb reacted more strongly than antigen IIa with all of the aspergilloma and allergic bronchopulmonary aspergillosis sera tested by enzyme-linked immunosorbent assay. The glycoprotein nature of antigen IIb was shown by the concanavalin A binding properties and staining reactions of the components.

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Year:  1983        PMID： 6347893      PMCID： PMC264698          DOI： 10.1128/iai.41.2.698-701.1983

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  17 in total

1.  Enzyme linked immunosorbent assay (ELISA) for IgG and IgE antibodies to protein and polysaccharide antigens of Aspergillus fumigatus.

Authors:  R Sepulveda; J L Longbottom; J Pepys
Journal:  Clin Allergy       Date:  1979-07

2.  The preparation and chemical composition of fractions from Aspergillus fumigatus wall and protoplasts possessing antigenic activity.

Authors:  V M Hearn; D W Mackenzie
Journal:  J Gen Microbiol       Date:  1979-05

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Glycoprotein staining following electrophoresis on acrylamide gels.

Authors:  R M Zacharius; T E Zell; J H Morrison; J J Woodlock
Journal:  Anal Biochem       Date:  1969-07       Impact factor: 3.365

5.  Identification and quantitation of antigens and antibodies by means of quantitative immunoelectrophoresis. A survey of methods.

Authors:  N H Axelsen; E Bock
Journal:  J Immunol Methods       Date:  1972-01       Impact factor: 2.303

6.  Isoelectric focusing in agarose.

Authors:  C A Saravis; N Zamcheck
Journal:  J Immunol Methods       Date:  1979       Impact factor: 2.303

7.  Antigenic and enzymatic architecture of Micrococcus lysodeikticus membranes established by crossed immunoelectrophoresis.

Authors:  P Owen; M R Salton
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205

8.  Galactomannan antigenemia in invasive aspergillosis.

Authors:  E Reiss; P F Lehmann
Journal:  Infect Immun       Date:  1979-07       Impact factor: 3.441

9.  Antigenic variability of Aspergillus fumigatus strains.

Authors:  V P Kurup; J N Fink; G H Scribner; M J Falk
Journal:  Microbios       Date:  1977

10.  Antigens of Micropolyspora faeni strains.

Authors:  V P Kurup; J N Fink
Journal:  Int Arch Allergy Appl Immunol       Date:  1979
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  6 in total

Review 1.  Serological techniques for diagnosis of fungal infection.

Authors:  L de Repentigny
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1989-04       Impact factor: 3.267

2.  Structural analysis of water-soluble fractions obtained from Aspergillus fumigatus mycelium.

Authors:  V M Hearn; B L Griffiths; P A Gorin
Journal:  Glycoconj J       Date:  1989       Impact factor: 2.916

3.  Antibody response to low-molecular-weight antigens of Aspergillus fumigatus in allergic bronchopulmonary aspergillosis.

Authors:  V P Kurup; P A Greenberger; J N Fink
Journal:  J Clin Microbiol       Date:  1989-06       Impact factor: 5.948

4.  Analysis of Aspergillus nidulans conidial antigens and their prevalence in other Aspergillus species.

Authors:  P Puente; M C Ovejero; N Fernández; F Leal
Journal:  Infect Immun       Date:  1991-12       Impact factor: 3.441

5.  Immunological characterization of Asp f 2, a major allergen from Aspergillus fumigatus associated with allergic bronchopulmonary aspergillosis.

Authors:  B Banerjee; P A Greenberger; J N Fink; V P Kurup
Journal:  Infect Immun       Date:  1998-11       Impact factor: 3.441

Review 6.  Immunodiagnosis of aspergillosis.

Authors:  V P Kurup; A Kumar
Journal:  Clin Microbiol Rev       Date:  1991-10       Impact factor: 26.132
  6 in total

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