| Literature DB >> 6345509 |
D O Wood, W H Atkinson, R S Sikorski, H H Winkler.
Abstract
Recombinant DNA techniques were used to isolate the Rickettsia prowazekii citrate synthase gene on the plasmid vector pBR322 by functional complementation of a gltA mutation of Escherichia coli K-12. Analysis of citrate synthase activity in crude extracts revealed that the enzyme expressed in E. coli retains the regulatory control mechanisms characteristic of the rickettsial enzyme.Entities:
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Year: 1983 PMID: 6345509 PMCID: PMC217695 DOI: 10.1128/jb.155.1.412-416.1983
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490