Literature DB >> 6345201

Dissociation of nucleoprotein complexes by chaotropic salts.

S Damodaran, J E Kinsella.   

Abstract

The effect of various anions in destabilizing yeast nucleoprotein complexes followed the order F- less than Cl- less than Br- less than ClO-4 congruent to Cl3CCOO-. Treatment of yeast nucleoproteins with 0.5 M NaClO4 resulted in removal of 80% of RNA. Based on the results, a simple method for effective separation of RNA from ribosomal particles is proposed and the mechanism of RNA dissociation by anions is also discussed.

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Year:  1983        PMID： 6345201     DOI： 10.1016/0014-5793(83)80675-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  3 in total

1.  Use of Cyclodextrin as a Novel Agent in the SEC-HPLC Mobile Phase to Mitigate the Interactions of Proteins or Peptide or their Impurities with the Residual Silanols of Commercial SEC-HPLC Columns with Improved Separation and Resolution.

Authors:  Indu Javeri; Kaliappanadar Nellaiappan; Charles McNemar; Kirill Yakovlevsky; Amina Soukrati; Phanindra Velisetty; Bijay Misra
Journal:  Pharm Res       Date:  2018-06-26       Impact factor: 4.200

2.  Single-Step Affinity Purification (ssAP) and Mass Spectrometry of Macromolecular Complexes in the Yeast S. cerevisiae.

Authors:  Christian Trahan; Marlene Oeffinger
Journal:  Methods Mol Biol       Date:  2022

3.  Antigenic properties of the SARS-CoV-2 nucleoprotein are altered by the RNA admixture.

Authors:  Denis E Kolesov; Maria V Sinegubova; Irina V Safenkova; Ivan I Vorobiev; Nadezhda A Orlova
Journal:  PeerJ       Date:  2022-01-07       Impact factor: 2.984
  3 in total

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