Literature DB >> 6344110

Determination of plasmid copy number by fluorescence densitometry.

S J Projan, S Carleton, R P Novick.   

Abstract

A simple and reliable method for the determination of plasmid copy numbers by direct fluorescence densitometry of ethidium bromide-stained electrophoretic gels was developed. In developing the method, the following parameters were evaluated and controlled: plasmid DNA trapping in the linear chromosomal DNA, staining-destaining kinetics for ethidium bromide, linearity of the fluorescence response, and the effect of the molecular topology of DNA on ethidium bromide binding to DNA in agarose.

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Year:  1983        PMID： 6344110     DOI： 10.1016/0147-619x(83)90019-7

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  93 in total

1.  Quantitation of supercoiled circular content in plasmid DNA solutions using a fluorescence-based method.

Authors:  M S Levy; P Lotfian; R O'Kennedy; M Y Lo-Yim; P A Shamlou
Journal:  Nucleic Acids Res       Date:  2000-06-15       Impact factor: 16.971

2.  Identification of an unknown promoter, OUTIIp, within the IS10R element.

Authors:  Esteban Martínez-García; Juana María Navarro-Lloréns; Antonio Tormo
Journal:  J Bacteriol       Date:  2003-03       Impact factor: 3.490

3.  Characterization of Lactococcus lactis UV-sensitive mutants obtained by ISS1 transposition.

Authors:  P Duwat; A Cochu; S D Ehrlich; A Gruss
Journal:  J Bacteriol       Date:  1997-07       Impact factor: 3.490

4.  An automated microplate-based method for monitoring DNA strand breaks in plasmids and bacterial artificial chromosomes.

Authors:  Cassandra Rock; Parviz Ayazi Shamlou; M Susana Levy
Journal:  Nucleic Acids Res       Date:  2003-06-01       Impact factor: 16.971

5.  The relapsing fever agent Borrelia hermsii has multiple copies of its chromosome and linear plasmids.

Authors:  T Kitten; A G Barbour
Journal:  Genetics       Date:  1992-10       Impact factor: 4.562

6.  Chi sequence protects against RecBCD degradation of DNA in vivo.

Authors:  P Dabert; S D Ehrlich; A Gruss
Journal:  Proc Natl Acad Sci U S A       Date:  1992-12-15       Impact factor: 11.205

7.  Mutations in bglY, the structural gene for the DNA-binding protein H1 of Escherichia coli, increase the expression of the kanamycin resistance gene carried by plasmid pGR71.

Authors:  P Bertin; P Lejeune; C Colson; A Danchin
Journal:  Mol Gen Genet       Date:  1992-05

8.  A DNA replication gene maps near terC in Escherichia coli K12.

Authors:  T Ben-Neria; E Z Ron
Journal:  Mol Gen Genet       Date:  1991-04

9.  Termination of DNA replication is required for cell division in Escherichia coli.

Authors:  N Grossman; E Rosner; E Z Ron
Journal:  J Bacteriol       Date:  1989-01       Impact factor: 3.490

10.  Mobilization of the relaxable Staphylococcus aureus plasmid pC221 by the conjugative plasmid pGO1 involves three pC221 loci.

Authors:  S J Projan; G L Archer
Journal:  J Bacteriol       Date:  1989-04       Impact factor: 3.490
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