Literature DB >> 6343790

Repair of gamma-ray induced DNA strand breaks in the radiation-sensitive mutant rad18-2 of Saccharomyces cerevisiae.

M R Mowat, W J Jachymczyk, P J Hastings, R C von Borstel.   

Abstract

The repair of gamma-ray induced DNA single and double-strand breaks was looked at in wild type and rad18-2 strains of the yeast Saccharomyces cerevisiae using sucrose gradient centrifugation. It was found that rad18-2 diploid cells could repair single and double-strand breaks induced by gamma-rays. It was also found that rad18-2 cells experienced a breakup of their DNA during post-irradiation incubation to a size smaller than seen in cells just receiving irradiation. This breakup of DNA in rad18-2 cells is not degradation due to cell death since wild type cells irradiated to similar low survival levels do not show this breakup of DNA with 8 h incubation. The breakup of DNA in rad18-2 cells is not due to replication gaps being formed by synthesis on a damaged template since treatment of rad18-2 a mating type cells with alpha factor, to prevent initiation of DNA synthesis, does not prevent breakup of the DNA.

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Year:  1983        PMID： 6343790     DOI： 10.1007/bf00337814

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  36 in total

1.  The role of bacteriophage T7 exonuclease (gene 6) in genetic recombination and production of concatemers.

Authors:  R C Miller; M Lee
Journal:  J Mol Biol       Date:  1976-02-25       Impact factor: 5.469

2.  A genetic study of x-ray sensitive mutants in yeast.

Authors:  J C Game; R K Mortimer
Journal:  Mutat Res       Date:  1974-09       Impact factor: 2.433

3.  DNA labelling and its assay in yeast.

Authors:  J Hatzfeld
Journal:  Biochim Biophys Acta       Date:  1973-02-23

4.  The petite mutation in yeast. Loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide.

Authors:  E S Goldring; L I Grossman; D Krupnick; D R Cryer; J Marmur
Journal:  J Mol Biol       Date:  1970-09-14       Impact factor: 5.469

5.  DNA synthesis in UV-irradiated yeast.

Authors:  L di Caprio; B S Cox
Journal:  Mutat Res       Date:  1981-06       Impact factor: 2.433

6.  Recombination in Saccharomyces cerevisiae: a DNA repair mutation associated with elevated mitotic gene conversion.

Authors:  W R Boram; H Roman
Journal:  Proc Natl Acad Sci U S A       Date:  1976-08       Impact factor: 11.205

7.  Lack of chemically induced mutation in repair-deficient mutants of yeast.

Authors:  L Prakash
Journal:  Genetics       Date:  1974-12       Impact factor: 4.562

8.  Metabolic suppressors of trimethoprim and ultraviolet light sensitivities of Saccharomyces cerevisiae rad6 mutants.

Authors:  C W Lawrence; R B Christensen
Journal:  J Bacteriol       Date:  1979-09       Impact factor: 3.490

9.  Characterization of the mutator mutation mut5-1.

Authors:  D P Morrison; P J Hastings
Journal:  Mol Gen Genet       Date:  1979-08

10.  Repair of interstrand cross-links in DNA of Saccharomyces cerevisiae requires two systems for DNA repair: the RAD3 system and the RAD51 system.

Authors:  W J Jachymczyk; R C von Borstel; M R Mowat; P J Hastings
Journal:  Mol Gen Genet       Date:  1981
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  7 in total

1.  Interchromosomal and intrachromosomal recombination in rad 18 mutants of Saccharomyces cerevisiae.

Authors:  R H Schiestl; R D Gietz; P J Hastings; U Wintersberger
Journal:  Mol Gen Genet       Date:  1990-06

2.  Isolation of the RAD18 gene of Saccharomyces cerevisiae and construction of rad18 deletion mutants.

Authors:  F Fabre; N Magana-Schwencke; R Chanet
Journal:  Mol Gen Genet       Date:  1989-02

3.  Repair of gamma ray-induced S1 nuclease hypersensitive sites in yeast depends on homologous mitotic recombination and a RAD18-dependent function.

Authors:  E M Geigl; F Eckardt-Schupp
Journal:  Curr Genet       Date:  1991-07       Impact factor: 3.886

4.  a/alpha-control of DNA repair in the yeast Saccharomyces cerevisiae: genetic and physiological aspects.

Authors:  M Heude; F Fabre
Journal:  Genetics       Date:  1993-03       Impact factor: 4.562

5.  The yeast rad18 mutator specifically increases G.C----T.A transversions without reducing correction of G-A or C-T mismatches to G.C pairs.

Authors:  B A Kunz; X L Kang; L Kohalmi
Journal:  Mol Cell Biol       Date:  1991-01       Impact factor: 4.272

6.  Repair of 8-methoxypsoralen photoinduced cross-links in yeast. Analysis by alkaline step-elution and electron microscopy.

Authors:  E Cundari; M Dardalhon; S Rousset; D Averbeck
Journal:  Mol Gen Genet       Date:  1991-09

7.  Enhanced genomic instability and defective postreplication repair in RAD18 knockout mouse embryonic stem cells.

Authors:  Satoshi Tateishi; Hitoshi Niwa; Jun-Ichi Miyazaki; Shiho Fujimoto; Hirokazu Inoue; Masaru Yamaizumi
Journal:  Mol Cell Biol       Date:  2003-01       Impact factor: 4.272
  7 in total

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