Literature DB >> 6343615

A routine flat embedding method for electron microscopy of microorganisms allowing selection and precisely orientated sectioning of single cells by light microscopy.

O L Reymond, J D Pickett-Heaps.   

Abstract

A simple method is described to embed material in resin, in the form of microscope slides, to observe it with high resolution light microscopy, to select, orient and section it for TEM. This method can be applied to many kinds of material but is particularly useful for the study of rare or tiny plant or animal microorganisms from field or culture. A diamond scriber, translucent hydrosoluble resin release agent, translucent and smooth resin stubs and a longitudinally perforated block-holder for ultramicrotome are the specific tools of this method.

Mesh：

Year:  1983        PMID： 6343615     DOI： 10.1111/j.1365-2818.1983.tb04200.x

Source DB:  PubMed          Journal:  J Microsc        ISSN： 0022-2720            Impact factor:   1.758


  10 in total

Review 1.  Out with the old and in with the new: rapid specimen preparation procedures for electron microscopy of sectioned biological material.

Authors:  Kent L McDonald
Journal:  Protoplasma       Date:  2013-11-21       Impact factor: 3.356

2.  Endocytic membrane fusion and buckling-induced microtubule severing mediate cell abscission.

Authors:  John A Schiel; Kristin Park; Mary K Morphew; Evan Reid; Andreas Hoenger; Rytis Prekeris
Journal:  J Cell Sci       Date:  2011-04-12       Impact factor: 5.285

3.  Carbonic anhydrase activity in primary sensory neurons. I. Requirements for the cytochemical localization in the dorsal root ganglion of chicken and mouse by light and electron microscopy.

Authors:  J Kazimierczak; E W Sommer; E Philippe; B Droz
Journal:  Cell Tissue Res       Date:  1986       Impact factor: 5.249

4.  Carbon partitioning to cellulose synthesis.

Authors:  C H Haigler; M Ivanova-Datcheva; P S Hogan; V V Salnikov; S Hwang; K Martin; D P Delmer
Journal:  Plant Mol Biol       Date:  2001-09       Impact factor: 4.076

5.  Inhibition of dendritic cell maturation by malaria is dose dependent and does not require Plasmodium falciparum erythrocyte membrane protein 1.

Authors:  Salenna R Elliott; Timothy P Spurck; Joelle M Dodin; Alexander G Maier; Till S Voss; Francisca Yosaatmadja; Paul D Payne; Geoffrey I McFadden; Alan F Cowman; Stephen J Rogerson; Louis Schofield; Graham V Brown
Journal:  Infect Immun       Date:  2007-04-30       Impact factor: 3.441

6.  Silver enhancement of Nanogold particles during freeze substitution for electron microscopy.

Authors:  M Morphew; W He; P J Bjorkman; J Richard McIntosh
Journal:  J Microsc       Date:  2008-05       Impact factor: 1.758

7.  The effects of chemical fixation on the cellular nanostructure.

Authors:  Yue Li; Luay M Almassalha; John E Chandler; Xiang Zhou; Yolanda E Stypula-Cyrus; Karl A Hujsak; Eric W Roth; Reiner Bleher; Hariharan Subramanian; Igal Szleifer; Vinayak P Dravid; Vadim Backman
Journal:  Exp Cell Res       Date:  2017-06-30       Impact factor: 3.905

8.  Nuclear migration in a nud mutant of Aspergillus nidulans is inhibited in the presence of a quantitatively normal population of cytoplasmic microtubules.

Authors:  S L Meyer; S G Kaminskyj; I B Heath
Journal:  J Cell Biol       Date:  1988-03       Impact factor: 10.539

9.  Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell.

Authors:  Diego Bohórquez; Fariha Haque; Satish Medicetty; Rodger A Liddle
Journal:  J Vis Exp       Date:  2015-07-19       Impact factor: 1.355

10.  All three components of the neuronal SNARE complex contribute to secretory vesicle docking.

Authors:  Yao Wu; Yiwen Gu; Mary K Morphew; Jun Yao; Felix L Yeh; Min Dong; Edwin R Chapman
Journal:  J Cell Biol       Date:  2012-08-06       Impact factor: 10.539
  10 in total

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