| Literature DB >> 6343497 |
J Carrere, R Grataroli, J Marfin, B Ferrua, J P Thouvenot, C Figarella.
Abstract
A sandwich enzyme immunoassay was developed for human pancreatic trypsin 1 using polystyrene balls coated with specific IgG as the first antibody and peroxidase-labeled IgG as the second antibody. The entire assay takes 6 h and the detection limit is 0.5 microgram/l. The assay can be performed on sera samples or on discs carrying dried blood spots. Good agreement was found with a radioimmunoassay kit. This simple assay could be widely applied to confirm the elevated immunoreactive serum trypsin described in newborn children with cystic fibrosis.Entities:
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Year: 1983 PMID： 6343497 DOI： 10.1016/0022-1759(83)90351-4
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303