Literature DB >> 6343428

Rapid, radiolabeled-microculture method that uses macrophages for in vitro evaluation of Mycobacterium leprae viability and drug susceptibility.

A Mittal, M Sathish, P S Seshadri, I Nath.   

Abstract

This paper describes a microculture rapid assay using radiolabeling and mouse macrophages to determine the viability and the drug susceptibility or resistance of Mycobacterium leprae. Comparison of M. leprae resident macrophage cultures maintained in 96-well flat-bottomed plates showed results for viability and susceptibility or resistance to dapsone that were similar to results for concurrent cultures in Leighton tubes with greater numbers of bacilli and macrophages.

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Year:  1983        PMID： 6343428      PMCID： PMC272723          DOI： 10.1128/jcm.17.4.704-707.1983

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  A method for counting acid-fast bacteria.

Authors:  C C Shepard; D H McRae
Journal:  Int J Lepr Other Mycobact Dis       Date:  1968 Jan-Mar

2.  Enhanced susceptibility of thymectomized and irradiated mice to infection with Mycobacterium leprae.

Authors:  R J Rees
Journal:  Nature       Date:  1966-08-06       Impact factor: 49.962

3.  Rapid, radiolabeled macrophage culture method for detection of dapsone-resistant Mycobacterium leprae.

Authors:  I Nath; H K Prasad; M Sathish; K V Desikan; P S Seshadri; C G Iyer
Journal:  Antimicrob Agents Chemother       Date:  1982-01       Impact factor: 5.191

4.  Lack of correlation between morphological index and viability as assessed by the uptake of 3H-thymidine by macrophage resident M. leprae.

Authors:  M Sathish; H K Prasad; A Mittal; I Nath
Journal:  Lepr India       Date:  1982-07

5.  Radiolabelled M. leprae resident in human macrophage cultures as an in vitro indicator of effective immunity in human leprosy.

Authors:  H K Prasad; R Singh; I Nath
Journal:  Clin Exp Immunol       Date:  1982-09       Impact factor: 4.330

6.  The uptake of 3H-thymidine in Mycobacterium leprae inoculated mouse macrophage cultures as a rapid indicator of bacillary viability. Factors influencing the specificity of the in vitro assay.

Authors:  M Sathish; I Nath
Journal:  Int J Lepr Other Mycobact Dis       Date:  1981-06

7.  Incorporation of 3H-thymidine in Mycobacterium leprae within differentiated human macrophages.

Authors:  H K Prasad; I Nath
Journal:  J Med Microbiol       Date:  1981-08       Impact factor: 2.472
  7 in total
  7 in total

1.  In vitro and in vivo activities of macrolides against Mycobacterium leprae.

Authors:  S G Franzblau; R C Hastings
Journal:  Antimicrob Agents Chemother       Date:  1988-12       Impact factor: 5.191

Review 2.  Leprosy.

Authors:  R C Hastings; T P Gillis; J L Krahenbuhl; S G Franzblau
Journal:  Clin Microbiol Rev       Date:  1988-07       Impact factor: 26.132

3.  In vitro effects of antimicrobial agents on Mycobacterium leprae in mouse peritoneal macrophages.

Authors:  N Ramasesh; J L Krahenbuhl; R C Hastings
Journal:  Antimicrob Agents Chemother       Date:  1989-05       Impact factor: 5.191

4.  Radiolabeling of Mycobacterium leprae lipids within schwannoma cells, a potential drug screening system.

Authors:  Y Mistry; N H Antia; R Mukherjee
Journal:  Antimicrob Agents Chemother       Date:  1991-07       Impact factor: 5.191

5.  Rapid in vitro metabolic screen for antileprosy compounds.

Authors:  S G Franzblau; R C Hastings
Journal:  Antimicrob Agents Chemother       Date:  1987-05       Impact factor: 5.191

6.  Effects of activated macrophages on Mycobacterium leprae.

Authors:  N Ramasesh; L B Adams; S G Franzblau; J L Krahenbuhl
Journal:  Infect Immun       Date:  1991-09       Impact factor: 3.441

7.  Alternate radiolabeled markers for detecting metabolic activity of Mycobacterium leprae residing in murine macrophages.

Authors:  H K Prasad; R C Hastings
Journal:  J Clin Microbiol       Date:  1985-05       Impact factor: 5.948
  7 in total

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