Literature DB >> 6342607

Fluorigenic method for the assay of proteinase activity with the use of 4-methylumbelliferyl-casein.

H Khalfan, R Abuknesha, D Robinson.   

Abstract

A method for the preparation of casein labelled with the 4-methylumbelliferyl fluorophore is described, and the product was used as a fluorigenic macromolecular substrate for a sensitive assay of the activity of proteinases. Nanogram quantities of trypsin, chymotrypsin, elastase and cathepsin D can be detected, but the substrate is unaffected by cathepsin B.

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Year:  1983        PMID: 6342607      PMCID: PMC1154083          DOI: 10.1042/bj2090265

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  6 in total

1.  Immunofluorimetric assay of oestradiol-17 beta.

Authors:  G I Ekeke; D Exley; R Abuknesha
Journal:  J Steroid Biochem       Date:  1979 Nov-Dec       Impact factor: 4.292

2.  Sensitive radiochemical assay for proteolytic activities.

Authors:  H Drucker
Journal:  Anal Biochem       Date:  1972-04       Impact factor: 3.365

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Sensitive assays for trypsin, elastase, and chymotrypsin using new fluorogenic substrates.

Authors:  M Zimmerman; B Ashe; E C Yurewicz; G Patel
Journal:  Anal Biochem       Date:  1977-03       Impact factor: 3.365

5.  A new assay for proteases using fluorescent labeling of proteins.

Authors:  R Wiesner; W Troll
Journal:  Anal Biochem       Date:  1982-04       Impact factor: 3.365

Review 6.  Control of proteolysis.

Authors:  H Holzer; P C Heinrich
Journal:  Annu Rev Biochem       Date:  1980       Impact factor: 23.643

  6 in total
  1 in total

1.  Aminomethyl coumarin acetic acid: a new fluorescent labelling agent for proteins.

Authors:  H Khalfan; R Abuknesha; M Rand-Weaver; R G Price; D Robinson
Journal:  Histochem J       Date:  1986-09
  1 in total

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