Literature DB >> 6339565

Immunoperoxidase techniques: the deleterious effect of sodium azide on the activity of peroxidase conjugates.

T C Richardson, D V Chapman, E Heyderman.   

Abstract

The effect of including sodium azide as a bacteriostatic agent in solutions used to dilute antibodies conjugated with the enzyme horseradish peroxidase was examined. An enzyme-linked immunosorbent assay (ELISA) and an immunohistochemical method were used and both techniques demonstrated an inhibitory effect of sodium azide on the activity of the peroxidase conjugates. It is concluded that the use of sodium azide in solutions used to dilute peroxidase conjugates is to be avoided.

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Year:  1983        PMID： 6339565      PMCID： PMC498236          DOI： 10.1136/jcp.36.4.411

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  4 in total

1.  THE OXIDATIC ACTIVITY OF HORSERADISH PEROXIDASE. I. OXIDATION OF HYDRO- AND NAPHTHOHYDROQUINONES.

Authors:  M H KLAPPER; D P HACKETT
Journal:  J Biol Chem       Date:  1963-11       Impact factor: 5.157

Review 2.  Immunoperoxidase technique in histopathology: applications, methods, and controls.

Authors:  E Heyderman
Journal:  J Clin Pathol       Date:  1979-10       Impact factor: 3.411

3.  A new sensitive colorimetric assay for peroxidase using 3,3'-diaminobenzidine as hydrogen donor.

Authors:  V Herzog; H D Fahimi
Journal:  Anal Biochem       Date:  1973-10       Impact factor: 3.365

4.  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors:  R C Graham; M J Karnovsky
Journal:  J Histochem Cytochem       Date:  1966-04       Impact factor: 2.479
  4 in total
  4 in total

1.  Deleterious effect of sodium azide on the activity of peroxidase.

Authors:  R K Chaudhary; C Parker
Journal:  J Clin Pathol       Date:  1983-10       Impact factor: 3.411

2.  Sodium azide induces necrotic cell death in rat squamous cell carcinoma SCC131.

Authors:  Eiju Sato; Toshimitsu Suzuki; Nobuo Hoshi; Takashi Sugino; Hiroshi Hasegawa
Journal:  Med Mol Morphol       Date:  2008-12-24       Impact factor: 2.309

3.  Twenty-sixth annual general meeting of the British Association for Cancer Research (in conjunction with the European Organization for Research and Treatment for Cancer--Pharmacokinetics and Metabolism Group and the Drug Metabolism Group). March 24-27, 1985, Birmingham, U.K.

Authors: 
Journal:  Br J Cancer       Date:  1985-09       Impact factor: 7.640

4.  A new monoclonal antibody to epithelial membrane antigen (EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2.

Authors:  E Heyderman; I Strudley; G Powell; T C Richardson; J L Cordell; D Y Mason
Journal:  Br J Cancer       Date:  1985-09       Impact factor: 7.640
  4 in total

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