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Literature DB >> 6338831

Detection of Giardia lamblia by immunofluorescence.

J L Riggs, K W Dupuis, K Nakamura, D P Spath.

Abstract

High-titer immune sera to cysts of Giardia lamblia, produced in guinea pigs, were labeled with fluorescein isothiocyanate. The resulting conjugates were used to detect G. lamblia in stool specimens by fluorescence microscopy. The sera also reacted with cysts of Chilomastix mesnili, but the two organisms could be differentiated by their size.

Entities: Chemical Disease Species

Mesh：

Substances：
Immune Sera

Year: 1983 PMID： 6338831 PMCID： PMC242346 DOI： 10.1128/aem.45.2.698-700.1983

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

4 in total

Review 1. Giardiasis.

Authors: E A Meyer; E L Jarroll
Journal: Am J Epidemiol Date: 1980-01 Impact factor: 4.897

2. Serum antibodies and jejunal histology in giardiasis associated with malabsorption.

Authors: M J Ridley; D S Ridley
Journal: J Clin Pathol Date: 1976-01 Impact factor: 3.411

3. An immunofluorescence test to detect serum antibodies to Giardia lamblia.

Authors: G S Visvesvara; P D Smith; G R Healy; W R Brown
Journal: Ann Intern Med Date: 1980-12 Impact factor: 25.391

4. Ultrastructure of the cyst of Giardia lamblia.

Authors: H G Sheffield; B Bjorvat
Journal: Am J Trop Med Hyg Date: 1977-01 Impact factor: 2.345

4 in total

12 in total

1. Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples.

Authors: A K Bej; M H Mahbubani; J L Dicesare; R M Atlas
Journal: Appl Environ Microbiol Date: 1991-12 Impact factor: 4.792

2. Immunofluorescence detection of Cryptosporidium oocysts in fecal smears.

Authors: H H Stibbs; J E Ongerth
Journal: J Clin Microbiol Date: 1986-10 Impact factor: 5.948

3. Pasteurization of naturally contaminated water with solar energy.

Authors: D A Ciochetti; R H Metcalf
Journal: Appl Environ Microbiol Date: 1984-02 Impact factor: 4.792

4. Evaluation of nine immunoassay kits (enzyme immunoassay and direct fluorescence) for detection of Giardia lamblia and Cryptosporidium parvum in human fecal specimens.

Authors: L S Garcia; R Y Shimizu
Journal: J Clin Microbiol Date: 1997-06 Impact factor: 5.948

Detection of Giardia lamblia by immunofluorescence.

Review 1. Giardiasis.

2. Serum antibodies and jejunal histology in giardiasis associated with malabsorption.

3. An immunofluorescence test to detect serum antibodies to Giardia lamblia.

4. Ultrastructure of the cyst of Giardia lamblia.

1. Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples.

2. Immunofluorescence detection of Cryptosporidium oocysts in fecal smears.

3. Pasteurization of naturally contaminated water with solar energy.

4. Evaluation of nine immunoassay kits (enzyme immunoassay and direct fluorescence) for detection of Giardia lamblia and Cryptosporidium parvum in human fecal specimens.

5. Comparison of two methods for detection of Giardia cysts and Cryptosporidium oocysts in water.

6. Detection of Giardia cysts by using the polymerase chain reaction and distinguishing live from dead cysts.

7. Comparison of techniques for detecting antigens of Giardia lamblia and Cryptosporidium parvum in faeces.

8. Detection of Giardia lamblia cysts in stool samples by immunofluorescence using monoclonal antibody.

9. Differentiation of Giardia duodenalis from other Giardia spp. by using polymerase chain reaction and gene probes.

10. Evaluation of a tangential-flow multiple-filter technique for detection of Giardia lamblia cysts in water.