Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Limitations of a fluorescence assay for studies on tetracycline transport into Escherichia coli.

Literature DB >> 6338818

Limitations of a fluorescence assay for studies on tetracycline transport into Escherichia coli.

Abstract

Transport of tetracycline into Escherichia coli was studied by two methods, one involving an absolute determination of accumulated drug and the other a fluorescence assay. Tetracycline uptake was nonsaturable when assayed by the absolute method, but fluorescence enhancement was maximal at an initial external tetracycline concentration of about 200 microM. The two transport assays also gave different results for the pH optimum of tetracycline transport. The absolute method indicated a pH optimum of 7.0 to 8.0, whereas the fluorescence method gave a value of 5.5 to 6.0. These data indicate that the fluorescence assay is of limited value in certain situations.

Entities: Chemical Species

Mesh：

Substances：
Tetracycline
Magnesium

Year: 1983 PMID： 6338818 PMCID： PMC184639 DOI： 10.1128/AAC.23.1.175

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

16 in total

1. Membrane phase transitions and the transport of chlortetracycline.

Authors: M E Dockter; J A Magnuson
Journal: Arch Biochem Biophys Date: 1975-05 Impact factor: 4.013

2. Accumulation of tetracyclines by Escherichia coli K-12.

Authors: P R Ball; I Chopra; S J Eccles
Journal: Biochem Biophys Res Commun Date: 1977-08-22 Impact factor: 3.575

3. Interaction of tetracylines with ribosomal subunits from Escherichia coli. A fluorometric investigation.

Authors: G Fey; M Reiss; H Kersten
Journal: Biochemistry Date: 1973-03-13 Impact factor: 3.162

4. Role of magnesium in the binding of tetracycline to Escherichia coli ribosomes.

Authors: J P White; C R Cantor
Journal: J Mol Biol Date: 1971-05-28 Impact factor: 5.469

5. Visualization of membrane bound cations by a fluorescent technique.

Authors: A H Caswell; J D Hutchison
Journal: Biochem Biophys Res Commun Date: 1971-01-08 Impact factor: 3.575

6. Plasmid-mediated tetracycline resistance in Escherichia coli involves increased efflux of the antibiotic.

Authors: P R Ball; S W Shales; I Chopra
Journal: Biochem Biophys Res Commun Date: 1980-03-13 Impact factor: 3.575

7. Characterization of the active transport of chlorotetracycline in staphylococcus aureus by a fluorescence technique.

Authors: M E Dockter; J A Magnuson
Journal: J Supramol Struct Date: 1974

8. Active accumulation of tetracycline by Escherichia coli.

Authors: T J Franklin; B Higginson
Journal: Biochem J Date: 1970-01 Impact factor: 3.857

9. Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli.

Authors: L McMurry; R E Petrucci; S B Levy
Journal: Proc Natl Acad Sci U S A Date: 1980-07 Impact factor: 11.205

Limitations of a fluorescence assay for studies on tetracycline transport into Escherichia coli.

1. Membrane phase transitions and the transport of chlortetracycline.

2. Accumulation of tetracyclines by Escherichia coli K-12.

3. Interaction of tetracylines with ribosomal subunits from Escherichia coli. A fluorometric investigation.

4. Role of magnesium in the binding of tetracycline to Escherichia coli ribosomes.

5. Visualization of membrane bound cations by a fluorescent technique.

6. Plasmid-mediated tetracycline resistance in Escherichia coli involves increased efflux of the antibiotic.

7. Characterization of the active transport of chlorotetracycline in staphylococcus aureus by a fluorescence technique.

8. Active accumulation of tetracycline by Escherichia coli.

9. Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli.

10. Membrane lateral phase separations and chlortetracycline transport by Bacillus megaterium.

1. Role of Staphylococcus aureus Tet38 in Transport of Tetracycline and Its Regulation in a Salt Stress Environment.

2. Tetracyclines of various hydrophobicities as a probe for permeability of Escherichia coli outer membranes.

3. Energetics of tetracycline transport into Escherichia coli.

4. Tetracycline uptake by susceptible Escherichia coli cells.

5. Subcellular Chemical Imaging of Antibiotics in Single Bacteria Using C₆₀-Secondary Ion Mass Spectrometry.