Literature DB >> 6338123

A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells.

J D Sedgwick, P G Holt.   

Abstract

A sensitive immunoassay is described for the detection of idiotype- and isotype-specific antibody-secreting cells (ASC), based upon the well established principles of ELISA. Single cell suspensions containing ASC are incubated on solid phase to which specific antigen has been chemically conjugated. Antibody attaches to the latter within the immediate microenvironment of the ASC, producing localized zones of bound antibody which are subsequently developed as visual 'spots' in the ELISA. This assay system has sensitivity and specificity at least equivalent to haemolytic plaque assays.

Mesh：

Year:  1983        PMID： 6338123     DOI： 10.1016/0022-1759(83)90091-1

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  132 in total

1.  Repeated administration of synthetic oligodeoxynucleotides expressing CpG motifs provides long-term protection against bacterial infection.

Authors:  D M Klinman; J Conover; C Coban
Journal:  Infect Immun       Date:  1999-11       Impact factor: 3.441

2.  The distribution of IgE plasma cells in lymphoid and non-lymphoid tissues of high-IgE responder rats: differential localization of antigen-specific and 'bystander' components of the IgE response to inhaled antigen.

Authors:  C McMenamin; B Girn; P G Holt
Journal:  Immunology       Date:  1992-12       Impact factor: 7.397

3.  T-cell-independent stimulation of immunoglobulin secretion in resting human B lymphocytes by the mannose-specific adhesin of Escherichia coli type 1 fimbriae.

Authors:  S Ponniah; S N Abraham; R O Endres
Journal:  Infect Immun       Date:  1992-12       Impact factor: 3.441

4.  Studies of immunity and bacterial invasiveness in mice given a recombinant salmonella vector encoding murine interleukin-6.

Authors:  S J Dunstan; A J Ramsay; R A Strugnell
Journal:  Infect Immun       Date:  1996-07       Impact factor: 3.441

5.  Stem cell factor enhances immunoglobulin E-dependent mediator release from cultured rat bone marrow-derived mast cells: activation of previously unresponsive cells demonstrated by a novel ELISPOT assay.

Authors:  P B Hill; A J MacDonald; E M Thornton; G F Newlands; S J Galli; H R Miller
Journal:  Immunology       Date:  1996-02       Impact factor: 7.397

6.  Alveolar macrophages down-regulate local pulmonary immune responses against intratracheally administered T-cell-dependent, but not T-cell-independent antigens.

Authors:  T Thepen; K Hoeben; J Brevé; G Kraal
Journal:  Immunology       Date:  1992-05       Impact factor: 7.397

7.  Heavy-chain isotype patterns of human antibody-secreting cells induced by Haemophilus influenzae type b conjugate vaccines in relation to age and preimmunity.

Authors:  T Barington; L Juul; A Gyhrs; C Heilmann
Journal:  Infect Immun       Date:  1994-08       Impact factor: 3.441

8.  Protection against infertility in a BALB/c mouse salpingitis model by intranasal immunization with the mouse pneumonitis biovar of Chlamydia trachomatis.

Authors:  S Pal; T J Fielder; E M Peterson; L M de la Maza
Journal:  Infect Immun       Date:  1994-08       Impact factor: 3.441

9.  Expansion of gamma interferon-producing CD8+ T cells following secondary infection of mice immune to Leishmania major.

Authors:  I Müller; P Kropf; J A Louis; G Milon
Journal:  Infect Immun       Date:  1994-06       Impact factor: 3.441

10.  Increased state of activation of CD4 positive T cells and elevated interferon gamma production in pouchitis.

Authors:  A Stallmach; F Schäfer; S Hoffmann; S Weber; I Müller-Molaian; T Schneider; G Köhne; K W Ecker; G Feifel; M Zeitz
Journal:  Gut       Date:  1998-10       Impact factor: 23.059
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