Literature DB >> 6337862

A microquantitative method for investigating the interaction between labeled lectins and the surface membranes of human lymphocytes using a semi-automatic harvesting machine.

M A Jacquet, A Sharif.   

Abstract

We describe a simple, rapid and economical method for the study of the interaction of labeled lectins and the surface membranes of human lymphocytes using a semi-automatic harvesting machine (Titertek Multiple Cell Harvester). The procedure requires both small numbers of cells and small amounts of lectin, moreover it reduces the number of experimental steps required.

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Year:  1983        PMID: 6337862     DOI: 10.1007/bf01960666

Source DB:  PubMed          Journal:  Experientia        ISSN: 0014-4754


  15 in total

1.  Human peripheral lymphocyte transformation induced by the Robina lectin.

Authors:  A Sharif; R Bourrillon
Journal:  Cell Immunol       Date:  1975-10       Impact factor: 4.868

2.  Modifications of lectin binding on human leukemic cells after neuraminidase treatment.

Authors:  A Sharif; J L Pico; C Choquet; C Rosenfeld; R Bourrillon
Journal:  Biomedicine       Date:  1978-04

Review 3.  The biochemistry of plant lectins (phytohemagglutinins).

Authors:  H Lis; N Sharon
Journal:  Annu Rev Biochem       Date:  1973       Impact factor: 23.643

Review 4.  Characteristics of antigenic determinants of intact cell surfaces.

Authors:  G I Pardoe; G Uhlenbruck
Journal:  J Med Lab Technol       Date:  1970-04

5.  Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

Authors:  A Böyum
Journal:  Scand J Clin Lab Invest Suppl       Date:  1968

6.  The nature of the erythrocyte receptor site for Robinia pseudoaccacia phytohemagglutinin.

Authors:  A M Leseney; R Bourrillon; S Kornfeld
Journal:  Arch Biochem Biophys       Date:  1972-12       Impact factor: 4.013

7.  Protein labeling by acetylation.

Authors:  I R Miller; H Great
Journal:  Biopolymers       Date:  1972       Impact factor: 2.505

8.  [Purification and physicochemical properties of a phytohemagglutinin from Robinia pseudoaccacia. Isolation of an active glycopeptide].

Authors:  R Bourrillon; J Font
Journal:  Biochim Biophys Acta       Date:  1968-01-22

9.  Reversible interaction of human lymphocytes with the mitogen concanavalin A.

Authors:  A E Powell; M A Leon
Journal:  Exp Cell Res       Date:  1970-10       Impact factor: 3.905

10.  Use of pliable bags in liquid scintillation counting.

Authors:  G A Simonnet; M A Jacquet; A Sharif; R Engler
Journal:  Int J Appl Radiat Isot       Date:  1981-08
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