Literature DB >> 6337548

Dual-substrate plate diffusion assay for proteases.

T J Montville.   

Abstract

A plate diffusion assay for endopeptidases was developed. Proteases applied to plates containing 1% casein, 1% gelatin, and 1.5% agar caused distinct zones reminiscent of immunoprecipitation bands. The diameter of the zones was linearly proportional to the log of the enzyme activity applied over a range from 0.01 to greater than 100 IU/ml.

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Year:  1983        PMID： 6337548      PMCID： PMC242253          DOI： 10.1128/aem.45.1.200-204.1983

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

1.  THE LOWRY MODIFICATION OF THE FOLIN REAGENT FOR DETERMINATION OF PROTEINASE ACTIVITY.

Authors:  C E MCDONALD; L L CHEN
Journal:  Anal Biochem       Date:  1965-01       Impact factor: 3.365

2.  The preparation and properties of two new chromogenic substrates of trypsin.

Authors:  B F ERLANGER; N KOKOWSKY; W COHEN
Journal:  Arch Biochem Biophys       Date:  1961-11       Impact factor: 4.013

3.  Rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on agar plates.

Authors:  H Kiyohara; K Nagao; K Yana
Journal:  Appl Environ Microbiol       Date:  1982-02       Impact factor: 4.792

4.  Isolation and characterization of a protease from Clostridium botulinum type B.

Authors:  B R Dasgupta; H Sugiyama
Journal:  Biochim Biophys Acta       Date:  1972-06-16

5.  Plate diffusion assay as a rapid method for dosimetry of mutagens.

Authors:  T E Awerbuch; A A Stark
Journal:  Appl Environ Microbiol       Date:  1979-12       Impact factor: 4.792

6.  Protease formation by a moderately halophilic Bacillus strain.

Authors:  M Kamekura; H Onishi
Journal:  Appl Microbiol       Date:  1974-04

7.  A fluorescent assay for proteolytic enzymes.

Authors:  C Schwabe
Journal:  Anal Biochem       Date:  1973-06       Impact factor: 3.365

8.  Effect of plating medium on heat activation requirement of Clostridium botulinum spores.

Authors:  T J Montville
Journal:  Appl Environ Microbiol       Date:  1981-10       Impact factor: 4.792
  8 in total
  14 in total

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Authors:  Z E Juarez; M W Stinson
Journal:  Infect Immun       Date:  1999-01       Impact factor: 3.441

2.  A culture-based method for determining the production of secreted protease inhibitors.

Authors:  David Quintero; David Bermudes
Journal:  J Microbiol Methods       Date:  2014-03-12       Impact factor: 2.363

3.  Enzymatic Activity and Susceptibility to Antifungal Agents of Brazilian Environmental Isolates of Hortaea werneckii.

Authors:  Andrea Formoso; Daiane Heidrich; Ciro Ramón Felix; Anne Carolyne Tenório; Belize R Leite; Danielle M Pagani; Santiago Ortiz-Monsalve; Mauricio Ramírez-Castrillón; Melissa Fontes Landell; Maria L Scroferneker; Patricia Valente
Journal:  Mycopathologia       Date:  2015-07-11       Impact factor: 2.574

4.  Characterization of a halo-acid-tolerant variant of Clostridium botulinum B-aphis.

Authors:  T J Montville
Journal:  Appl Environ Microbiol       Date:  1984-08       Impact factor: 4.792

5.  Native isolate of Trichoderma: a biocontrol agent with unique stress tolerance properties.

Authors:  N Mishra; S S Khan; S Krishna Sundari
Journal:  World J Microbiol Biotechnol       Date:  2016-06-23       Impact factor: 3.312

6.  New protease mutants in Aspergillus niger result in strongly reduced in vitro degradation of target proteins; genetical and biochemical characterization of seven complementation groups.

Authors:  J P van den Hombergh; P J van de Vondervoort; N C van der Heijden; J Visser
Journal:  Curr Genet       Date:  1995-09       Impact factor: 3.886

7.  Dependence of Clostridium botulinum gas and protease production on culture conditions.

Authors:  T J Montville
Journal:  Appl Environ Microbiol       Date:  1983-02       Impact factor: 4.792

8.  Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis.

Authors:  J A Wells; E Ferrari; D J Henner; D A Estell; E Y Chen
Journal:  Nucleic Acids Res       Date:  1983-11-25       Impact factor: 16.971

9.  Inhibition of Clostridium botulinum 52A toxicity and protease activity by sodium acid pyrophosphate in media systems.

Authors:  M K Wagner; F F Busta
Journal:  Appl Environ Microbiol       Date:  1985-07       Impact factor: 4.792

10.  Efficient polygalacturonase production from agricultural and agro-industrial residues by solid-state culture of Aspergillus sojae under optimized conditions.

Authors:  Doreen Heerd; Sonja Diercks-Horn; Marcelo Fernández-Lahore
Journal:  Springerplus       Date:  2014-12-16
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