Literature DB >> 6337369

Expression of hepatitis B surface antigen gene in yeast.

A Miyanohara, A Toh-e, C Nozaki, F Hamada, N Ohtomo, K Matsubara.   

Abstract

The DNA sequence coding for hepatitis B virus surface antigen (HBsAg) was placed under control of the repressible acid phosphatase promoter of the yeast Saccharomyces cerevisiae in a plasmid capable of autonomous replication in both yeast and Escherichia coli. Yeast transformed by this plasmid synthesized up to 5 X 10(5) molecules per cell of immunologically active HBsAg polypeptide in phosphate-free medium. The HBsAg polypeptides produced in the yeast cells were assembled into 20- to 22-nm spherical or oval particles and were immunogenic.

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Year:  1983        PMID： 6337369      PMCID： PMC393297          DOI： 10.1073/pnas.80.1.1

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

1.  In vitro construction of different oligomeric forms of lambdadv DNA and studies of their transforming activities.

Authors:  K Matsubara; Y Takagi; T Mukai
Journal:  J Virol       Date:  1975-09       Impact factor: 5.103

2.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

3.  Isolation and characterization of acid phosphatase mutants in Saccharomyces cerevisiae.

Authors:  A To-E; Y Ueda; S I Kakimoto; Y Oshima
Journal:  J Bacteriol       Date:  1973-02       Impact factor: 3.490

4.  Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors:  D B Clewell
Journal:  J Bacteriol       Date:  1972-05       Impact factor: 3.490

5.  Purification of naked intranuclear particles from human liver infected by hepatitis B virus.

Authors:  S Z Hirschman; M Gerber; E Garfinkel
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205

6.  Australia antigen: large-scale purification from human serum and biochemical studies of its proteins.

Authors:  J L Gerin; P V Holland; R H Purcell
Journal:  J Virol       Date:  1971-05       Impact factor: 5.103

7.  Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.

Authors:  C C Richardson
Journal:  Proc Natl Acad Sci U S A       Date:  1965-07       Impact factor: 11.205

8.  A gene controlling the synthesis of non specific alkaline phosphatase in Saccharomyces cerevisiae.

Authors:  A Toh-E; H Nakamura; Y Oshima
Journal:  Biochim Biophys Acta       Date:  1976-03-25

9.  Regulation and characterization of acid and alkaline phosphatase in yeast.

Authors:  A Schurr; E Yagil
Journal:  J Gen Microbiol       Date:  1971-03

10.  Functional expression of cloned yeast DNA in Escherichia coli.

Authors:  B Ratzkin; J Carbon
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205
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  23 in total

1.  Analysis of human T-cell antigen receptor variable beta gene usage following vaccination with recombinant HBsAg.

Authors:  K Yuh; S Sugyo; K Nakamura; H Shijo; K Emi; K Harada; S Yoshitake; N Kimura; T Moribe; T Kaneshige; M Okumura
Journal:  Dig Dis Sci       Date:  1998-04       Impact factor: 3.199

Review 2.  The Density Code for the Development of a Vaccine?

Authors:  Wei Cheng
Journal:  J Pharm Sci       Date:  2016-09-17       Impact factor: 3.534

3.  Bacterial identification using nitrocellulose blotting technique that incorporates ELISA to bacterial surface antigen.

Authors:  T T Hansel; N Matthews
Journal:  J Clin Pathol       Date:  1987-02       Impact factor: 3.411

4.  Hepatitis B virus surface antigen assembly function persists when entire transmembrane domains 1 and 3 are replaced by a heterologous transmembrane sequence.

Authors:  Ira Berkower; Angelo Spadaccini; Hong Chen; Danah Al-Awadi; Jacqueline Muller; Yamei Gao; Dino Feigelstock; Konstantin Virnik; Yisheng Ni
Journal:  J Virol       Date:  2010-12-22       Impact factor: 5.103

5.  End Joining-Mediated Gene Expression in Mammalian Cells Using PCR-Amplified DNA Constructs that Contain Terminator in Front of Promoter.

Authors:  Mikiko Nakamura; Ayako Suzuki; Junko Akada; Keisuke Tomiyoshi; Hisashi Hoshida; Rinji Akada
Journal:  Mol Biotechnol       Date:  2015-12       Impact factor: 2.695

6.  Recombinant hepatitis B surface antigen and anionic phospholipids share a binding region in the fifth domain of beta2-glycoprotein I (apolipoprotein H).

Authors:  Haider Mehdi; Asma Naqvi; M Ilyas Kamboh
Journal:  Biochim Biophys Acta       Date:  2008-01-12

7.  Interferon regulatory factor-1 (IRF-1) activates the synthetic IRF-1-responsive sequence (GAAAGT)4 in Saccharomyces cerevisiae.

Authors:  A Sailer; K Nagata; D Näf; M Aebi; C Weissmann
Journal:  Gene Expr       Date:  1992

8.  Optimization of the expression system using galactose-inducible promoter for the production of anticoagulant hirudin in Saccharomyces cerevisiae.

Authors:  E S Choi; J H Sohn; S K Rhee
Journal:  Appl Microbiol Biotechnol       Date:  1994-12       Impact factor: 4.813

9.  Saccharomyces cerevisiae can release hepatitis B virus surface antigen (HBsAg) particles into the medium by its secretory apparatus.

Authors:  S Kuroda; T Miyazaki; S Otaka; Y Fujisawa
Journal:  Appl Microbiol Biotechnol       Date:  1993-11       Impact factor: 4.813

10.  Effect of bumetanide on toluene diisocyanate induced contractions in guinea pig airways.

Authors:  C E Mapp; A Boniotti; A Papi; C A Maggi; A Di Stefano; M Saetta; A Ciaccia; L M Fabbri
Journal:  Thorax       Date:  1993-01       Impact factor: 9.139
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