Literature DB >> 6334360

Homologous recombination catalyzed by mammalian cell extracts in vitro.

V Darby, F Blattner.   

Abstract

An assay was developed to detect recombination events taking place in an in vitro reaction. Extracts of cultured mouse preB lymphocytes were found to catalyze homologous recombination between substrate DNA molecules but not site-specific recombination between cloned mouse immunoglobulin D and J genes. Addition of deoxyribonucleoside triphosphates increased the frequency of homologous recombination. This recombination activity was not observed in two differentiated lymphocyte cell lines.

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Year:  1984        PMID： 6334360     DOI： 10.1126/science.6334360

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  11 in total

1.  Heteroduplex DNA formation is associated with replication and recombination in poxvirus-infected cells.

Authors:  C Fisher; R J Parks; M L Lauzon; D H Evans
Journal:  Genetics       Date:  1991-09       Impact factor: 4.562

2.  Recombination between irradiated shuttle vector DNA and chromosomal DNA in African green monkey kidney cells.

Authors:  J S Mudgett; W D Taylor
Journal:  Mol Cell Biol       Date:  1990-01       Impact factor: 4.272

3.  Characterization of nonconservative homologous junctions in mammalian cells.

Authors:  L Desautels; S Brouillette; J Wallenburg; A Belmaaza; N Gusew; P Trudel; P Chartrand
Journal:  Mol Cell Biol       Date:  1990-12       Impact factor: 4.272

4.  Characterization of an ATP-dependent DNA strand transferase from human cells.

Authors:  D Ganea; P Moore; L Chekuri; R Kucherlapati
Journal:  Mol Cell Biol       Date:  1987-09       Impact factor: 4.272

5.  Extrachromosomal recombination in mammalian cells as studied with single- and double-stranded DNA substrates.

Authors:  F L Lin; K M Sperle; N L Sternberg
Journal:  Mol Cell Biol       Date:  1987-01       Impact factor: 4.272

6.  Homologous recombination intermediates between two duplex DNA catalysed by human cell extracts.

Authors:  B Lopez; S Rousset; J Coppey
Journal:  Nucleic Acids Res       Date:  1987-07-24       Impact factor: 16.971

7.  DNA mismatch repair detected in human cell extracts.

Authors:  P M Glazer; S N Sarkar; G E Chisholm; W C Summers
Journal:  Mol Cell Biol       Date:  1987-01       Impact factor: 4.272

8.  Effect of marker distance and orientation on recombinant formation in poxvirus-infected cells.

Authors:  R J Parks; D H Evans
Journal:  J Virol       Date:  1991-03       Impact factor: 5.103

9.  Repair of deletions and double-strand gaps by homologous recombination in a mammalian in vitro system.

Authors:  R Jessberger; P Berg
Journal:  Mol Cell Biol       Date:  1991-01       Impact factor: 4.272

10.  General recombination mechanisms in extracts of meiotic cells.

Authors:  Y Hotta; S Tabata; R A Bouchard; R Piñon; H Stern
Journal:  Chromosoma       Date:  1985       Impact factor: 4.316
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