Literature DB >> 6334116

Purification and characterization of human, guinea pig and mouse C1q by fast protein liquid chromatography (FPLC).

F Stemmer, M Loos.   

Abstract

A simple and rapid procedure for the purification of C1q from human, guinea pig and mouse serum is described. This procedure allows the purification of C1q within one and a half days using euglobulin precipitation, chromatography on Superose 6B, followed by chromatography on Mono S by Fast Protein Liquid Chromatography (FPLC). The highly purified, hemolytically active C1q is free of any immunoglobulins. Since the purification of C1q of three different species was performed by the same purification procedure, a comparison of the subunit compositions was made under reducing and non-reducing conditions on SDS-PAGE. The yield was found to be more than 50%.

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Year:  1984        PMID： 6334116     DOI： 10.1016/0022-1759(84)90361-2

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  8 in total

1.  [Autoantibodies against the complement component C1q in systemic lupus erythematosus].

Authors:  U Antes; H P Heinz; K Hartung; M Loos
Journal:  Klin Wochenschr       Date:  1990-11-09

2.  Contributions of C1q, bacterial lipopolysaccharide, and porins during attachment and ingestion phases of phagocytosis by murine macrophages.

Authors:  B Euteneuer; S Störkel; M Loos
Journal:  Infect Immun       Date:  1986-03       Impact factor: 3.441

3.  C1q-bearing immune complexes detected by a monoclonal antibody to human C1q in rheumatoid arthritis sera and synovial fluids.

Authors:  U Antes; H P Heinz; D Schultz; D Brackertz; M Loos
Journal:  Rheumatol Int       Date:  1991       Impact factor: 2.631

4.  Humoral autoreactivity directed against surfactant protein-A (SP-A) in rheumatoid arthritis synovial fluids.

Authors:  P K Trinder; T P Hickling; R B Sim; D Brackertz; M Loos; M J Maeurer
Journal:  Clin Exp Immunol       Date:  2000-04       Impact factor: 4.330

5.  Autoreactivity to mouse C1q in a murine model of SLE.

Authors:  P K Trinder; M J Maeurer; H U Schorlemmer; M Loos
Journal:  Rheumatol Int       Date:  1995       Impact factor: 2.631

6.  Interaction of purified lipoteichoic acid with the classical complement pathway.

Authors:  M Loos; F Clas; W Fischer
Journal:  Infect Immun       Date:  1986-09       Impact factor: 3.441

7.  The complement system of the goat: haemolytic assays and isolation of major proteins.

Authors:  Isabel Moreno-Indias; Alister W Dodds; Anastasio Argüello; Noemi Castro; Robert B Sim
Journal:  BMC Vet Res       Date:  2012-06-26       Impact factor: 2.741

8.  Complement Protein C1q Binds to Hyaluronic Acid in the Malignant Pleural Mesothelioma Microenvironment and Promotes Tumor Growth.

Authors:  Chiara Agostinis; Romana Vidergar; Beatrice Belmonte; Alessandro Mangogna; Leonardo Amadio; Pietro Geri; Violetta Borelli; Fabrizio Zanconati; Francesco Tedesco; Marco Confalonieri; Claudio Tripodo; Uday Kishore; Roberta Bulla
Journal:  Front Immunol       Date:  2017-11-20       Impact factor: 7.561
  8 in total

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