Literature DB >> 6334017

T-lymphocyte progenitors in man: biochemical characterization of a colony promoting activity (CPA) active on immature precursors.

F Triebel, J C Gluckman, P Debre, D J Charron.   

Abstract

PHA-stimulated lymphocyte supernatants (P-SUP) containing colony promoting activity (CPA) and interleukin-2 (IL-2) were found to drive T-lymphocyte precursor cells (OKT4-OKT8- blood cells) into colonies of mature T cells in agar. Ammonium sulphate precipitation followed by gel filtration on Sephadex G-75 showed that both IL-2 activity and CPA coeluted in the 21,000 MW fraction. Chromatofocusing on a PBE 94 gel equilibrated between pH 7.4 and 4.2 showed that CPA coeluted with the two main peaks of IL-2 (pI 7.2, pI 6.7). Adsorption of IL-2 activity by more than 97% on different T-cell lines did not completely abolish CPA. These results suggest that other(s) factor(s) with the same biochemical characteristics as IL-2 play a role in the induction of growth and differentiation of immature T-cell precursors.

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Year:  1984        PMID： 6334017      PMCID： PMC1454888     

Source DB:  PubMed          Journal:  Immunology        ISSN： 0019-2805            Impact factor:   7.397


  12 in total

1.  Growth at limiting dilution of human T cell colonies from T cell-depleted peripheral blood leukocytes.

Authors:  J F Moreau; R G Miller
Journal:  J Immunol       Date:  1983-03       Impact factor: 5.422

2.  Characterization of the T lymphocyte colony-forming cells and evidence for the acquisition of T cell markers in the absence of the thymic microenvironment in man.

Authors:  F Triebel; W A Robinson; A R Hayward; P G de Laforest
Journal:  J Immunol       Date:  1981-05       Impact factor: 5.422

3.  Heterogeneity of human T-cell growth factor(s) due to variable glycosylation.

Authors:  R J Robb; K A Smith
Journal:  Mol Immunol       Date:  1981-12       Impact factor: 4.407

4.  Distribution of t lymphocyte subsets in the human bone marrow and thymus: an analysis with monoclonal antibodies.

Authors:  G Janossy; N Tidman; E S Papageorgiou; P C Kung; G Goldstein
Journal:  J Immunol       Date:  1981-04       Impact factor: 5.422

5.  Spontaneous release of a factor with properties of T cell growth factor from a continuous line of primate tumor T cells.

Authors:  H Rabin; R F Hopkins; F W Ruscetti; R H Neubauer; R L Brown; T G Kawakami
Journal:  J Immunol       Date:  1981-11       Impact factor: 5.422

6.  Chromatofocusing as a tool for the characterization and partial purification of human interleukin-2.

Authors:  J P Gérard; J Bertoglio
Journal:  J Immunol Methods       Date:  1982-12-17       Impact factor: 2.303

7.  Biochemical characterization of lymphocyte regulatory molecules. II. Purification of a class of rat and human lymphokines.

Authors:  S Gillis; K A Smith; J Watson
Journal:  J Immunol       Date:  1980-04       Impact factor: 5.422

8.  Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage.

Authors:  E L Reinherz; P C Kung; G Goldstein; R H Levey; S F Schlossman
Journal:  Proc Natl Acad Sci U S A       Date:  1980-03       Impact factor: 11.205

9.  The in vitro generation and sustained culture of nude mouse cytolytic T-lymphocytes.

Authors:  S Gillis; N A Union; P E Baker; K A Smith
Journal:  J Exp Med       Date:  1979-06-01       Impact factor: 14.307

10.  T cell growth factor receptors. Quantitation, specificity, and biological relevance.

Authors:  R J Robb; A Munck; K A Smith
Journal:  J Exp Med       Date:  1981-11-01       Impact factor: 14.307
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  2 in total

1.  Identification and purification of human T-lymphocyte colony-enhancing factor, TLCEF: increased production by phorbol myristate acetate.

Authors:  M M Werber; J Goldman; J Radnay; S Klein; L A Rozenszajn
Journal:  Immunology       Date:  1985-10       Impact factor: 7.397

2.  T-lymphocyte progenitors in man: phenotypic characterization of blood and bone marrow T-colony forming cells.

Authors:  F Triebel; J C Gluckman; F Chapuis; D Charron; P Debre
Journal:  Immunology       Date:  1985-02       Impact factor: 7.397
  2 in total

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