| Literature DB >> 6333465 |
W R Freeman, D H Char, M R Garavoy, H Lyon.
Abstract
Flow cytometric analysis of monoclonal antibody labelled lymphocytes is an important tool in understanding the immune response. Microtechniques were developed to analyze lymphocytes in small amounts of ocular fluids. Optimal labelling volumes and concentrations for Leu 2a, Leu 3a, Leu 4 and Leu M3 antibodies were determined. The ability to label and reproducibly analyze cell samples with as few as 1000 cells is demonstrated.Entities:
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Year: 1984 PMID： 6333465 DOI： 10.1016/0022-1759(84)90420-4
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303