Literature DB >> 6332819

Lymphokine purification by reversed-phase high-performance liquid chromatography.

D L Urdal, D Mochizuki, P J Conlon, C J March, M L Remerowski, J Eisenman, C Ramthun, S Gillis.   

Abstract

Reversed-phase high-performance liquid chromatography has been used to purify to homogeneity two different lymphokines. Human IL-2 was purified on a C8 reversed-phase column in pyridine-acetate-propanol followed by chromatography on a C18 reversed-phase column in trifluoroacetic acid-acetonitrile. Protein sequence analysis of in situ-generated cyanogen bromide peptides obtained from this preparation established the homogeneity of this material and confirmed the amino acid sequence predicted from the published DNA sequence. Murine CSF-2 alpha was purified on a C18 reversed-phase column in trifluoroacetic acid-acetonitrile followed by chromatography on the same column in pyridine-acetate-propanol. The final preparation yielded a single band on a sodium dodecyl sulfate-polyacrylamide gel with a molecular weight of 24,500.

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Year:  1984        PMID： 6332819     DOI： 10.1016/s0021-9673(01)96410-6

Source DB:  PubMed          Journal:  J Chromatogr


  16 in total

1.  Biochemical properties of chimeric skeletal and smooth muscle myosin light chain kinases.

Authors:  S A Leachman; P J Gallagher; B P Herring; M J McPhaul; J T Stull
Journal:  J Biol Chem       Date:  1992-03-05       Impact factor: 5.157

2.  Purification and chemical characterization of the receptor for interleukin 2 from activated human T lymphocytes and from a human T-cell lymphoma cell line.

Authors:  D L Urdal; C J March; S Gillis; A Larsen; S K Dower
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

3.  Interleukin-1 administration to C3H/HeJ mice after but not prior to infection increases resistance to Salmonella typhimurium.

Authors:  P J Morrissey; K Charrier
Journal:  Infect Immun       Date:  1991-12       Impact factor: 3.441

4.  Interleukin 1 regulates hematopoietic activity, a role previously ascribed to hemopoietin 1.

Authors:  D Y Mochizuki; J R Eisenman; P J Conlon; A D Larsen; R J Tushinski
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

5.  Characterization of the high-affinity cell-surface receptor for murine B-cell-stimulating factor 1.

Authors:  L S Park; D Friend; K Grabstein; D L Urdal
Journal:  Proc Natl Acad Sci U S A       Date:  1987-03       Impact factor: 11.205

6.  Molecular characterization of murine and human OX40/OX40 ligand systems: identification of a human OX40 ligand as the HTLV-1-regulated protein gp34.

Authors:  P R Baum; R B Gayle; F Ramsdell; S Srinivasan; R A Sorensen; M L Watson; M F Seldin; E Baker; G R Sutherland; K N Clifford
Journal:  EMBO J       Date:  1994-09-01       Impact factor: 11.598

7.  Characterization of the cell surface receptor for human granulocyte/macrophage colony-stimulating factor.

Authors:  L S Park; D Friend; S Gillis; D L Urdal
Journal:  J Exp Med       Date:  1986-07-01       Impact factor: 14.307

8.  Regulation of cytolytic cell populations from human peripheral blood by B cell stimulatory factor 1 (interleukin 4).

Authors:  M B Widmer; R B Acres; H M Sassenfeld; K H Grabstein
Journal:  J Exp Med       Date:  1987-11-01       Impact factor: 14.307

9.  Interleukin 7 promotes long-term in vitro growth of antitumor cytotoxic T lymphocytes with immunotherapeutic efficacy in vivo.

Authors:  D H Lynch; R E Miller
Journal:  J Exp Med       Date:  1994-01-01       Impact factor: 14.307

10.  Detection and characterization of high affinity plasma membrane receptors for human interleukin 1.

Authors:  S K Dower; S R Kronheim; C J March; P J Conlon; T P Hopp; S Gillis; D L Urdal
Journal:  J Exp Med       Date:  1985-08-01       Impact factor: 14.307
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