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Literature DB >> 6331541

NMR study of the exchange rates of allosterically responsive labile protons in the heme pockets of hemoglobin A.

Abstract

1H NMR spectroscopy has been used to measure the proximal histidyl labile ring proton (NH) rates of exchange with bulk solvent in the individual subunits of hemoglobin (Hb) A. These protons displayed a substantial decrease in their exchange rates in comparison with related monomeric proteins and exhibited sensitivity to the quarternary state. With the beta subunit NH, the exchange behaviour was similar to an allosterically responsive subset of protons, which have been identified using 1H-3H methods (Englander, J.J., R. Rogero, and S. W. Englander, 1983, J. Mol. Biol. 169:325-344). Assuming similar exchange mechanisms for the two subunits, the NMR data suggested a more flexible alpha than beta subunit in Hb A.

Entities: Chemical

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Year: 1984 PMID： 6331541 PMCID： PMC1434941 DOI： 10.1016/S0006-3495(84)84004-7

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

21 in total

1. STUDIES ON THE STRUCTURE OF HEMOGLOBIN. III. PHYSICOCHEMICAL PROPERTIES OF RECONSTITUTED HEMOGLOBINS.

Authors: E ANTONINI; M BRUNORI; A CAPUTO; E CHIANCONE; A R FANELLI; J WYMAN
Journal: Biochim Biophys Acta Date: 1964-03-30

2. Measurement of structural and free energy changes in hemoglobin by hydrogen exchange methods.

Authors: S W Englander
Journal: Ann N Y Acad Sci Date: 1975-04-15 Impact factor: 5.691

3. Assignment of proximal histidine proton NMR peaks in myoglobin and hemoglobin.

Authors: G N La Mar; D L Budd; H Goff
Journal: Biochem Biophys Res Commun Date: 1977-07-11 Impact factor: 3.575

4. Properties of the T state of human oxyhemoglobin studies by laser photolysis.

Authors: C A Sawicki; Q H Gibson
Journal: J Biol Chem Date: 1977-11-10 Impact factor: 5.157

Review 5. Hydrogen exchange kinetics and internal motions in proteins and nucleic acids.

Authors: C K Woodward; B D Hilton
Journal: Annu Rev Biophys Bioeng Date: 1979

6. Hydrogen exchange kinetics of human hemoglobins. The pH dependence of solvent accessibility in cyanomet-, oxy-, and deoxyhemoglobin.

Authors: B E Hedlund; P E Hallaway; B E Hallaway; E S Benson; A Rosenberg
Journal: J Biol Chem Date: 1978-05-25 Impact factor: 5.157

Review 7. Hydrogen exchange.

Authors: S W Englander; N W Downer; H Teitelbaum
Journal: Annu Rev Biochem Date: 1972 Impact factor: 23.643

8. The reaction of n-butyl isocyanide with human hemoglobin. II. The ligand-binding properties of the and chains within deoxyhemoglobin.

Authors: J S Olson; Q H Gibson
Journal: J Biol Chem Date: 1972-03-25 Impact factor: 5.157